表没食子儿茶素没食子酸酯体外α-葡萄糖苷酶活性的影响  

Effect of epigallocatechin-3-gallate on the activity of α-glucosidase in vitro

作　　者：祝宇铭[1] 丁红[1] 王竹[2] 杨月欣[2] 向雪松[2] 

机构地区：[1]新疆医科大学公共卫生学院营养与食品卫生学教研室,乌鲁木齐830011 [2]中国疾病预防控制中心营养与食品安全所

出　　处：《卫生研究》2010年第2期168-171,176,共5页Journal of Hygiene Research

基　　金：国家"十一五"科技支撑计划项目(No.2006BAD27B01)

摘　　要：目的研究酵母和大鼠小肠黏膜来源的α-葡萄糖苷酶活性测定的体外体系,评价在该体系条件下表没食子儿茶素没食子酸酯(EGCG)的α-葡萄糖苷酶抑制活性(AGI)的活性,为建立AGI活性评价体系提供依据。方法分别应用酵母和大鼠小肠黏膜提取液作为α-葡萄糖苷酶,以麦芽糖为底物;用0.00、0.25、0.50、1.0和1.50mg/ml的酶与0.00、0.01、0.02、0.04、0.06、0.08、0.1和0.12mol/l的底物反应,采用葡萄糖氧化酶法测定葡萄糖生成量来确定反应条件;在该反应条件下比较两种酶源的活性差异,并以此分析EGCG的AGI作用和计算半数抑制浓度(IC50)。结果最佳反应条件为0.50mg/ml的酶与0.06mol/l的底物反应,研究发现在该体系下EGCG对酵母来源的AGI活性强于小肠黏膜来源的酶,其IC50值分别为:0.150和0.837mg/ml。结论建立体外AGI活性评价体系有利于准确筛选AGI活性物质,酵母来源的酶可用于AGI活性的初步筛选;离体组织测定法可用于进一步评价AGI活性物质;EGCG体外具有较强的AGI活性。Objective To investigate the effect of epigallocatechin-3-gallate （ EGCG） on the activity of α- glucosidase derived from bakers’yeast and small intestinal mucosa of Wistar rats,in order to establish an evaluation system for the activity of α-glucosidase inhibitor. Methods α-glucosidase was prepared from the bakers’yeast and the small intestinal mucosa of Wistar rats. The inhibitory activity of EGCG on α-glucosidase was based on the concentration of glucose in the reaction system. Results EGCG was shown to be an α-glucosidase inhibitor. IC50 was 0. 150mg /ml and 0. 837mg /ml for α-glucosidase derived from bakers ’ yeast and small intestinal mucosa respectively. Conclusion The establishment of an in vitro evaluation system for AGI activity is conductive for screening α-glucosidase inhibitory substance. The AGI activity of EGCG is strong in vitro.

关 键 词：表没食子儿茶素没食子酸酯 最佳反应体系 降糖机制 Α-葡萄糖苷酶 

分 类 号：Q591.4[生物学—生物化学] Q556.2