纳豆激酶的亲和吸附工艺研究  

Adsorption of Nattokinase by Soybean Microspheres

作　　者：马跃华[1,2] 董超[2] 杨明[1,2] 史延茂[2] 

机构地区：[1]河北工业大学化工学院生物工程系,天津300130 [2]河北省生物研究所,河北石家庄050081

出　　处：《大豆科学》2010年第2期306-309,314,共5页Soybean Science

基　　金：河北省科技支撑计划资助项目(08276413D)

摘　　要：采用静态吸附方法验证了大豆颗粒对纳豆激酶的亲和吸附特性,测定了其静态吸附动力学特性和吸附等温线以及一些吸附条件。结果表明:该亲和吸附等温线符合Langmiur方程,吸附动力学符合扩散方程;吸附的最佳缓冲液选择pH6.0、0.01 mol.L-1的PBS,在静态时选用大豆颗粒的最大吸附量为6351.58 IU.g-1,洗脱后收率达到81.30%,纯化倍数约30.23倍。初步推断大豆蛋白中含有与纳豆激酶特异性吸附的的配体结构。The affinity adsorption of Nattokinase by soybean microspheres was characterized and the static adsorption dynamics, adsorption isotherms and some adsorption conditions were investigated in the present work. The results revealed that the adsorption isotherm was accorded with Langmuir adsorption isotherm and the adsorption dynamics was coincident with diffusion equation. The best buffer was 0.01 mol·L^-1PBS with pH of 6.0. Under this condition, the maximum adsorption capacity was 6 351.58 IU·g^-1. The purification fold and recovery of Nattokinase activity reached 30.23 and 81.3% ,respectively. It may be presumed that the soybean protein contains the ligand which absorb the nattokinase specifically.

关 键 词：大豆颗粒 纳豆激酶 吸附平衡 吸附动力学 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]