^(60)Co γ射线对糖多孢红霉菌的重复诱变研究  

Repeated mutagenic effects of ^(60)Co γ-rays on Saccharopolyspora erythraea

作　　者：武杉杉[1,2] 刘超[2] 张部昌[1] 曹磊[2] 汪晓鸣[2] 

机构地区：[1]安徽大学生命科学学院,合肥230039 [2]安徽省农业科学院农产品加工研究所,合肥230031

出　　处：《辐射研究与辐射工艺学报》2010年第2期103-106,共4页Journal of Radiation Research and Radiation Processing

基　　金：安徽省科技厅科研项目(08020303057);国家自然科学基金(30670068)资助

摘　　要：糖多孢红霉菌963是60Co γ射线对糖多孢红霉菌M诱变筛选获得的具有抑菌活性的突变株。本实验采用60Co γ射线对糖多孢红霉菌963进行诱变,研究了重复诱变效应。结果表明,各个辐照剂量下,突变株963的致死率均低于出发菌株M。重复诱变后,菌株的形态变异率达24.1‰,比以M菌株作为出发菌株的单次诱变提高了4.8倍。实验获得了1株产物抑菌活性增强的突变株,其抑菌活性比菌株963提高了40%,正突变率为2.01‰,比单次诱变提高了20.36%。采用重复诱变的方法,总体变异丰富,菌株的形态变异率和正突变率显著提高,有利于提高诱变育种工作的筛选效率。Saccharopolyspora erythraea 963 with antibacterial activity was a mutant strain which obtained by 60Co γ-rays irradiation on Saccharopolyspora erythraea M. In this experiment,Saccharopolyspora erythraea 963 was also radiated by 60Co γ-rays to study repeated mutagenic effects. The results indicate that the lethal effect of strain 963 is lower than that of starting strain M in the same absorbed doses of irradiation. After the repeated mutagenesis,the rate of morphological variation of strains was up to 24.1‰ and equal to 4.8 times as that of starting strain M. The inhibitory effect of positively mutated strain increased by 40% and the rate of positive mutation was 2.01‰ and rose by 20.36% compared with that of the strain 963. The method of repeated mutagenesis is helpful in improving screen-ing efficiency of mutation breeding as the morphological variation of mutated strain and positive mutation can be ob-tained.

关 键 词：60Co Γ射线 糖多孢红霉菌 诱变效应 

分 类 号：TS202.3[轻工技术与工程—食品科学] Q939.92[轻工技术与工程—食品科学与工程]