蔗糖酯脂质体对巨噬细胞迁移性能的影响  

Influence of sucrose ester phospholipids liposome on the migration of rabbit peritoneal macrophages

作　　者：陈淑芬[1] 姚震[1] 钱士匀[1] 钟江华[1] 伍强[1] 

机构地区：[1]海南医学院附属医院临床中心实验室,海南海口570102

出　　处：《中国热带医学》2010年第5期568-570,共3页China Tropical Medicine

基　　金：海南省自然科学基金资助课题(编号:30214)

摘　　要：目的观察蔗糖酯脂质体对家兔腹腔巨噬细胞内胆固醇及膜磷脂含量变化的影响,及其所导致的巨噬细胞迁移性能的变化。方法在家兔腹腔巨噬细胞与Ox-LDL共同孵育前后两种情况下,加入蔗糖酯-卵磷脂及卵磷脂脂质体进行干预,然后观测细胞内胆固醇、细胞膜磷脂及细胞迁移性能的变化。结果家兔腹腔巨噬细胞与Ox-LDL共同孵育24h后,其细胞内胆固醇含量及膜磷脂含量都明显增加,细胞的迁移性能则受到损伤。加入六种脂质体后,各组细胞的细胞内胆固醇含量无明显降低,但细胞的膜磷脂均有所降低,细胞的迁移性能严重受损;在加入Ox-LDL的同时加入脂质体,各组细胞的细胞内胆固醇含量均无明显增加,但经两种卵磷脂脂质体作用过的细胞的膜磷脂含量高于经四种蔗糖酯-卵磷脂脂质体作用过的细胞,同时细胞仍保持较好的迁移性能。结论蔗糖酯及卵磷脂脂质体可使泡沫化的巨噬细胞的迁移性能遭受进一步的损伤;但能阻碍巨噬细胞摄取Ox-LDL,并使细胞保持一定的迁移性能。Aim To observe the effect of liposome composed by sucrose ester and phospholipids on the change of intracellular cholesterol and membrane phospholipids in rabbit peritoneal macrophages. Methods The liposomes composed with lecithin and the sucrose ester-phospholipids were incubated with rabbit peritoneal macrophages after or simultaneously with the addition of ox-LDL,and the intracellular cholesterol and the membrane phospholipids content and the macrophage migration performance was assayed. Results The average intracellular cholesterol content and membrane phospholipids content of rabbit peritoneal macrophages was increased significantly after incubation with Ox-LDL for 24 hours,and the cell migration was impaired. Though the intracellular cholesterol content was not lowered after treatment by any of the six kinds of liposome,the membrane phospholipids content was decreased,and the cell migration was impaired heavily（24 hours）. Adding various kinds of liposome when the cells were incubated simultaneously with ox-LDL,the average intracellular cholesterol content did not significantly increase,but the phospholipids contents of the cells treated with two kinds of lecithin liposome were higher than that of the cells treated with four kinds of macrophages. Meanwhile,the cell migration remained a good performance after treated with two kinds of lecithin liposome for 24 hours. Conclusion The sucrose ester and lecithin liposomes may resutl in further damage to the migration capacity of froth macrophage, but can hinder the taking of ox-LDL by macrophage and help cell maintain certain migration performance.

关 键 词：蔗糖酯 脂质体 胆固醇 细胞膜磷脂 细胞迁移 

分 类 号：R-33[医药卫生]