培养基组成对米曲霉产氨基酰化酶的影响  

Effect of Culture Medium Components on Aminoacylase Production by Aspergillus Oryzae

作　　者：王霞[1] 刘丽华[1] 张霞[1] 王祎[1] 张同祥[1] 

机构地区：[1]内蒙古工业大学化工学院,内蒙古呼和浩特010051

出　　处：《化学与生物工程》2010年第4期67-70,共4页Chemistry & Bioengineering

基　　金：内蒙古自然科学基金资助项目(200711020206)

摘　　要：氨基酰化酶是一类能够水解N-酰基-DL-氨基酸上酰胺键的酶。通过单因素实验和正交实验,对米曲霉产氨基酰化酶的培养基组成进行了优化,以提高其产量。确定较优培养基组成为:麸皮2%、尿素1.5%、磷酸二氢钠0.3%、硫酸镁0.35%、Tween-80 0.1%,在此培养基上得到的氨基酰化酶单位酶活可提高到440.51μmol.g-1.h-1。The aminoacylase is a kind of enzyme,by which N-acetyl-DL-amino acid is hydrolyzed to give the D-or L-amino acid. Through single factor experiment and orthogonal experiment, the activity of aminoacylase production by Aspergillus oryzae was improved ,and the optimum culture medium components were obtained as followsbran 2%, urea 1.5%, NaH2PO4 0.3% , MgSO4 0.35% and Tween-80 0.1% . Under above conditions, the highest aminoacylase activity of 440.51 μmol·g^-1·h^-1 was obtained.

关 键 词：米曲霉 氨基酰化酶 正交实验 培养基 

分 类 号：TQ925[轻工技术与工程—发酵工程] TS201.3