Effects of sevoflurane preconditioning and postconditioning on rat myocardial stunning in ischemic reperfusion injury  

作　　者：An-lu DAI Li-hua FAN Feng-jiang ZHANG Mei-juan YANG Jing YU Jun-kuan WANG Tao FANG Gang CHEN Li-na YU Min YAN 

机构地区：[1]Department of Anesthesiology, the Second Affiliated Hospital School of Medicine, Zhejiang University, Hangzhou 310009, China [2]Department of Anesthesiology, Lishui People's Hospital, Lishui 323600, China [3]Department of Anesthesiology, Jinhua People's Hospital, Jinhua 321300, China

出　　处：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2010年第4期267-274,共8页浙江大学学报（英文版）B辑（生物医学与生物技术）

基　　金：Project supported by the National Natural Science Foundation of China (No. 30772090);the Natural Science Foundation of Zhejiang Province (No. Y204141);the Foundation from Science and Tech-nology Department of Zhejiang Province (No. 2007R10034);the Foundation from the Health Bureau of Zhejiang Province (No. 2007QN007), China

摘　　要：Ischemic preconditioning and postconditioning distinctly attenuate ventricular arrhythmia after ischemia without affecting the severity of myocardial stunning. Therefore, we report the effects of sevofiurane preconditioning and postconditioning on stunned myocardium in isolated rat hearts. Isolated rat hearts were underwent 20 min of global ischemia and 40 min of reperfusion. After an equilibration period （20 min）, the hearts in the preconditioning group were exposed to sevoflurane for 5 min and next washout for 5 min before ischemia. Hearts in the sevoflurane postconditioning group underwent equilibration and ischemia, followed immediately by sevoflurane exposure for the first 5 min of reperfusion. The control group received no treatment before and after ischemia. Left ventricular pressure, heart rate, coronary flow, electrocardiogram, and tissue histology were measured as variables of ventricular function and cellular injury, respectively. There was no significant difference in the duration of reperfusion ventricular arrhythmias between control and sevoflurane preconditioning group （P=0.195）. The duration of reperfusion ventricular arrhythmias in the sevoflurane postconditioning group was significantly shorter than that in the other two groups （P〈0.05）. ＋（dPIdt）max in the sevoflurane preconditioning group at 5, 10, 15, 20, and 30 min after reperfusion was significantly higher than that in the control group （P〈0.05）, and there were no significant differences at 40 min after reperfusion among the three groups （P〉0.05）. As expected, for a 20-min general ischemia, infarct size in heart slices determined by 2,3,5-triphenyltetrazolium chloride staining among the groups was not obvious. Sevofiurane postconditioning reduces reperfusion arrhythmias without affecting the severity of myocardial stunning. In contrast, sevoflurane preconditioning has no beneficial effects on reperfusion arrhythmias, but it is in favor of improving ventricular function and recovering myocardial stunning. SevofIschemic preconditioning and postconditioning distinctly attenuate ventricular arrhythmia after ischemia without affecting the severity of myocardial stunning. Therefore,we report the effects of sevoflurane preconditioning and postconditioning on stunned myocardium in isolated rat hearts. Isolated rat hearts were underwent 20 min of global ischemia and 40 min of reperfusion. After an equilibration period (20 min),the hearts in the preconditioning group were exposed to sevoflurane for 5 min and next washout for 5 min before ischemia. Hearts in the sevoflurane postconditioning group underwent equilibration and ischemia,followed immediately by sevoflurane exposure for the first 5 min of reperfusion. The control group received no treatment before and after ischemia. Left ventricular pressure,heart rate,coronary flow,electrocardiogram,and tissue histology were measured as variables of ventricular function and cellular injury,respectively. There was no significant difference in the duration of reperfusion ventricular ar-rhythmias between control and sevoflurane preconditioning group (P=0.195). The duration of reperfusion ventricular arrhythmias in the sevoflurane postconditioning group was significantly shorter than that in the other two groups (P<0.05). ±(dP/dt)max in the sevoflurane preconditioning group at 5,10,15,20,and 30 min after reperfusion was sig-nificantly higher than that in the control group (P<0.05),and there were no significant differences at 40 min after reperfusion among the three groups (P>0.05). As expected,for a 20-min general ischemia,infarct size in heart slices determined by 2,3,5-triphenyltetrazolium chloride staining among the groups was not obvious. Sevoflurane postcon-ditioning reduces reperfusion arrhythmias without affecting the severity of myocardial stunning. In contrast,sevoflu-rane preconditioning has no beneficial effects on reperfusion arrhythmias,but it is in favor of improving ventricular function and recovering myocardial stunning. Sevoflurane preconditioning and postconditioning

关 键 词：Inhalation anesthetics SEVOFLURANE POSTCONDITIONING PRECONDITIONING Ischemia-reperfusion injury Myocardial stunning 

分 类 号：R614[医药卫生—麻醉学]