花椰菜花叶病毒CaMV 35S启动子LAMP检测方法的建立  

Detection of CaMV 35S promoter by loop-mediated isothermal amplification

作　　者：孙敏[1] 梁成珠[1] 高宏伟[1] 林超[1] 刘彩霞[1] 

机构地区：[1]山东出入境检验检疫局,青岛266002

出　　处：《食品科技》2010年第4期255-258,共4页Food Science and Technology

摘　　要：针对花椰菜花叶病毒(CaMV)35S启动子的保守序列设计特异性引物,并进行条件优化,建立CaMV35S启动子的LAMP检测方法。该方法具有良好的特异性,检测灵敏度可达0.002%。对80份各类植物及其加工品进行检测,检测结果与实时荧光PCR法完全相符。CaMV35S启动子的LAMP检测方法,在检验检疫行业和食品加工业具有广阔的应用前景,对于加强转基因产品检测监管,促进进出口贸易具有重要意义。After designing the primers and optimizing experimental parameter,a LAMP assay for detecting 35S promoter of cauliflower mosaic virus was established.These primers were proved to be specific and the detective limit was 0.002%.The detection results of 80 samples showed that the positive rate was in agreement with real time PCR assay.The LAMP assay for detecting CaMV 35S promoter may provide a potential tool for products quarantine and food processing,which is of importance in supervising of genetically modified organisms and promoting exportation.

关 键 词：花椰菜花叶病毒 35S启动子 环介导等温扩增技术 检测 

分 类 号：TS207.3[轻工技术与工程—食品科学]