小鼠输精管模型法检测大豆蛋白酶解液外啡肽的研究  

Detecting Exorphins in Hydrolysate of Soybean Protein by Mouse Vas Difference in Vitro

作　　者：孙冰玉[1] 石彦国[1] 胡春林[1,2] 刘海波[1] 

机构地区：[1]哈尔滨商业大学食品工程学院,哈尔滨150076 [2]哈高科大豆食品有限责任公司,哈尔滨150078

出　　处：《中国粮油学报》2010年第4期16-19,共4页Journal of the Chinese Cereals and Oils Association

基　　金：国家科技支撑计划(2006BAD05A08)

摘　　要：外啡肽隐藏在一些食物蛋白分子序列的片段中,可通过适当的酶解作用释放出来。采用生物法检测评价大豆蛋白酶酶解液中产生外啡肽的情况。结果表明,经复合酶处理的大豆蛋白酶解液产生了阿片活性,其阿片活性能被纳洛酮(一种特异性阿片拮抗剂)所逆转,说明酶解液中产生了外啡肽物质,经胃蛋白蛋酶和Alcalase水解的样品酶解液产生外啡肽的半数抑制IC_(50)为0.410 mg/mL,纳络酮逆转效应为(0.153±0.018)mg/mL,为全面客观地评价大豆肽类食品提供科学依据,为外啡肽及其他有害肽类在大豆肽食品安全领域的进一步研究抛砖引玉。Exorphins, which hide in protein sequence fragment of some foods, may be released by enzymolysis function. Bioassay was used to detect the possibility of existence of exorphins in the hydrolysate of soybean protein. Results show that opioid activity exists and can be reversed by naloxon hydrochloride （a pure opioid receptor antagonist）. It is indicated that exorphins exists in the hydrolysate of soybean protein, the IC50 is 0.4mg/mL and the inversion effect of naloxon hydrochloride is 0. 153 mg/mL. The detection provides a scientific basis for appraising soybean peptide foods objectively and comprehensively.

关 键 词：生物法 外啡肽 阿片活性 纳洛酮 

分 类 号：TS21[轻工技术与工程—粮食、油脂及植物蛋白工程]