人参蛋白四种提取方法的比较研究  

Study on comparison of four kinds of extracting methods of ginseng protein

作　　者：王伟楠[1] 赵雨[1] 李红艳[1] 张惠[1] 幺宝金[2] 

机构地区：[1]长春中医药大学新药研发中心,吉林长春130117 [2]吉林大学公共卫生学院,吉林长春130021

出　　处：《食品工业科技》2010年第5期280-281,共2页Science and Technology of Food Industry

基　　金：国家自然科学基金项目(30873371);国家科技支撑计划(2007BAI38B02)

摘　　要：通过对人参蛋白的四种提取方法(盐析法、丙酮沉淀法、聚乙二醇沉淀法、中空纤维膜过滤法)进行比较,采用SDS-PAGE电泳法、Bradford蛋白纯度检测法获得相关数据。结果表明,聚乙二醇沉淀法和中空纤维膜过滤法收率最高;中空纤维膜过滤法与盐析法纯度最好;中空纤维膜过滤法的电泳条带最完整清晰。The comparative study was made between different extracting methods（salt out,acetone precipitation,PEG precipitation and hollow fiber membrane filtration）of ginseng protein with data which was attained by SDS-PAGE and Bradford protein assay knit. The results pointed out that the methods of PEG and hollow fiber membrane got the highest yield;hollow fiber membrane and salt out got the best purity;the SDS-PAGE band of hollow fiber membrane was the most tidy and clear.

关 键 词：人参蛋白 提取方法 SDS-PAGE电泳法 Bradford法 

分 类 号：TS201.2[轻工技术与工程—食品科学]