桐粕降解菌DBTM6培养条件及降解酶活性的研究  

Study on Culture Conditions of Disintegrator Bacteria of Tung Fructification DBTM6 and Enzyme Activity of Tung Fructification

作　　者：周毅峰[1] 邓桂芳[1] 秦恩华[1] 

机构地区：[1]湖北民族学院生物科学与技术学院,湖北恩施445000

出　　处：《湖北民族学院学报（自然科学版）》2010年第1期13-17,共5页Journal of Hubei Minzu University(Natural Science Edition)

基　　金：生物资源保护与利用湖北省重点实验室(湖北民族学院)资助项目(2007025);湖北民族学院生物科学与技术学院院内项目

摘　　要：采用单因素试验法对桐粕降解菌DBTM6菌发酵的主要影响因素碳源、氮源、温度、转速、初始pH值等进行了研究,通过正交试验对其发酵培养基在摇瓶阶段进行了优化,并在优化后的条件下测定了该菌的生长曲线及其产生的蛋白酶、果胶酶和纤维素酶的活性.结果表明:该菌株最适培养基各成分质量分数为:玉米粉2.0%,蛋白胨0.5%,NaCl 0.5%,最适条件:温度为40℃,pH为6.0,转速为200 r/min.在最适培养条件下测定了DBTM6菌的纤维素酶活力为3.5717 U/mL,果胶酶活力为1.1415 U/mL,蛋白酶活力为36.358 3 U/mL,比活力分别为13.8545U/mg,4.4279 U/mg,141.0331 U/mg.The fermentation of tung fructification DBTM6 was investigated through single-factor test.The main factors include：carbon,nitrogen,temperature,rotate speed and initial pH.The orthogonal experiment was used to optimize the medium,and then the growth curve and protease,pectinase and cellulase activity of the bacteria were detected in the optimized condition.The results showed that the suitable medium contained（g/mL）：corn power 2.0,tryptone 0.5,NaCl 0.5,the optimal fermentation conditions are： temperature 40℃,initial pH6.0,200r/min.Under optimum culture conditions,the cellulase activity is 3.5717U/mL,the pectinase activity is 1.1415U/mL,the protease activity is 36.3583 U/ mL,the activity were 13.8545,4.4279,141.0331U/mg,respectively.

关 键 词：桐粕降解菌 培养基优化 正交试验 降解酶活性 

分 类 号：Q93-335[生物学—微生物学] Q936