核桃仁种皮多糖的提取及抑菌作用的研究  

Extraction and Antimicrobial Activities of Polysaccharide from Walnut Kernel Pellicle

作　　者：刘安军[1] 王玥玮[1] 朱振元[1] 王妍[1] 陈影[1] 

机构地区：[1]天津科技大学食品工程与生物技术学院,天津300457

出　　处：《现代食品科技》2010年第4期362-365,369,共5页Modern Food Science and Technology

摘　　要：本文以0.25mol/L的NaOH-乙醇溶液作提取剂,从核桃仁种皮中提取多糖,提取液经去脂、去蛋白、醇析后得粗多糖WKPP-70。利用DEAE-Sephadex A-25凝胶柱和Sephadex G-75凝胶柱对粗多糖进行分离纯化,得纯多糖WKPP-70-1-1。通过紫外光谱分析证明其纯度,红外光谱分析显示其具有多糖类的特征吸收峰。抑菌试验表明,WKPP-70-1-1对大肠杆菌、沙门氏菌、枯草芽孢杆菌、金黄葡萄球菌均有一定的抑制作用。Crude polysaccharide, WKPP-70, was extracted from walnut kernel pellicle, using 0.25 mol/L NaOH-ethanol. After removal of lipids and proteins, WKPP-70 was further purified by chromatography on DEAE-Sephadex A-25 and Sephadex G-75 columns. The purity of the obtained polysaccharide, named as WKPP-70-1-1 was determined using UV spectrophotometric method and its structure was analyzed by infrared spectrum （IR）. The antimicrobial activities of WKPP-70-1-1 against E.scherichia coli, Bacillussubtilis, Staphy lococcus aureus and SalmonellaTyphimurium were also evaluated. The results showed that WKPP-70-1-1 had antimicrobial activities against these examined microorganisms.

关 键 词：核桃仁种皮 多糖 提取 纯化 抑菌作用 

分 类 号：TQ461[化学工程—制药化工]