高效液相色谱-串联质谱法测定酸奶中纳他霉素的含量  

Determination of natamycin in yoghurt using liquid chromatography-mass spectrometry

作　　者：李媛媛[1] 王伟[1] 关树文[2] 李刚[1] 董巧红[1] 

机构地区：[1]吉林省产品质量监督检验院,长春130022 [2]吉林大学生命科学学院,长春130021

出　　处：《中国卫生检验杂志》2010年第4期761-762,共2页Chinese Journal of Health Laboratory Technology

基　　金：科学前沿与交叉学科创新项目(200903261)

摘　　要：目的：利用液相色谱-串联质谱联用仪,建立酸奶中纳他霉素的液相色谱-串联质谱（LC-MS/MS）测定方法。方法：酸奶样品用乙醇超声波振荡提取,离心后,上清液待测。采用LC-MS/MS测定,色谱柱为C18反相色谱柱,流动相为乙腈-0.2%甲酸水溶液,电喷雾正离子模式电离,质谱多反应选择离子检测（MRM）,检测离子对为m/z 666→504和m/z 666→486,其中m/z 666→504为定量离子对,外标法定量。结果：纳他霉素线性相关系数为0.9994,不同水平的回收率为93.4%~101.7%,检出限为10μg/kg。结论：该法简便快速、准确可靠。Objective：To establish a liquid chromatography-mass spectrometry（LC-MS/MS） method for determination of natamycin in yoghurt.Methods：Natamycin in yoghurt was extracted by sonication with ethanol.The extract was centrifugalized.The separation of natamycin was performed on a C18 reversephase column using acetonitrile-0.2% formic acid as the mobile phase.The analysis of natamycin was carried out under positive ESI MS/MS and multiple reaction monitoring mode（MRM） by selecting m/z 666→486 as qualitative ions,and m/z 666→504 as quantitative ions.Results：The correlation coefficients were above 0.9994.Under the optimal conditions,the spiked recoveries of natamycin were in the range of 93.4%～101.7%.The detection limit was 10 μg/kg.Conclusion：The method has been proved to be simple and accurate.

关 键 词：纳他霉素 液相色谱-串联质谱 酸奶 多反应监测 

分 类 号：O657.6[理学—分析化学]