EGCG抑制肝癌细胞株HepG2增殖及HIF-1α/VEGF的表达  

Inhibitory effect of EGCG on proliferation and HIF-1α/VEGF expression in cell line HepG2

作　　者：赵志成[1] 庄莉[1] 贾长库[1] 郑树森[1] 

机构地区：[1]浙江大学医学院附属第一医院肝胆胰外科,卫生部多器官移植重点实验室,浙江省器官移植重点实验室,浙江杭州310003

出　　处：《中国病理生理杂志》2010年第4期713-720,共8页Chinese Journal of Pathophysiology

基　　金：浙江省中医药课题资助项目(No.2005C175);浙江省中医药重大课题资助项目(No.2007ZA014)

摘　　要：目的:研究表没食子儿茶素没食子酸酯(EGCG)抑制肝癌生长的分子机制。方法:采用MTT方法观察EGCG对HepG2细胞株增殖的影响;利用体外厌氧培养技术,通过不同剂量的EGCG进行干预,检测肝癌细胞HIF-1α/VEGF的基因转录和蛋白表达;将肝癌细胞种植于裸鼠体内,观察EGCG对肿瘤生长的抑制作用,并且测定HIF-1α/VEGF的表达。结果:体外试验显示EGCG对肝癌细胞株HepG2的增殖有明显抑制作用;明显下调HIF-1α/VEGF蛋白的表达,而对HIF-1α基因转录影响不大;裸鼠体内试验中观察到EGCG对肿瘤生长有明显抑制作用,抑制率达39.8%±5.1%。结论:EGCG可明显抑制肝癌细胞的增殖,干预HIF-1α/VEGF信号转导通路可能是其抑制肿瘤生长的主要机制之一。AIM：To study the molecular mechanism of EGCG on inhibiting the growth of hepatic carcinoma. METHODS： The proliferation of hepatic cell line HepG2 cultured with different doses of EGCG was studied by MTT and suspension/adherence methods. The effect of EGCG on the expression of HIF-1α/VEGF at mRNA and protein levels in vitro and in vivo was evaluated by RT-PCR and Western blotting,respectively. The inhibition of EGCG on the growth of tumor implanted into athymic nude mice was also observed. RESULTS： The proliferation of hepatic cell line HepG2 was inhibited by EGCG in a dose-dependent manner. The expression of HIF-1α/VEGF was suppressed markedly by EGCG at protein level. However,the inhibitory effect of EGCG on the mRNA expression was only observed on VEGF,not on HIF-1α. In the animal experiment,the implanted tumor growth was inhibited by 39.8%±5.1%. CONCLUSION： EGCG suppresses the hepatic carcinoma cell growth,and interrupts the HIF-1α/VEGF signaling pathway significantly,indicating a fundamental mechanism of EGCG for inhibiting tumor growth.

关 键 词：表没食子儿茶素没食子酸酯 肝肿瘤 缺氧诱导因子-1 血管内皮生长因子 

分 类 号：R735.7[医药卫生—肿瘤]