胶原蛋白的高效制备及与明胶的鉴别  

Development of Efficient Preparation and Identification Methods for Bovine Collagen

作　　者：汤俊[1] 魏东芝[1] 张国钧 沈菊泉[3] 董莹[1] 周文瑜[1] 马志英[3] 沈亚领[1] 

机构地区：[1]华东理工大学生物工程学院,生物反应器工程国家重点实验室,上海200237 [2]上海恰尔生物技术有限公司,上海201103 [3]上海市食品研究所,上海200235

出　　处：《食品科学》2010年第7期54-60,共7页Food Science

基　　金：“上海市重点学科”建设项目(B505);上海市科学技术委员会应用技术开发专项资金项目(05-022)

摘　　要：在分析酸提取法和酶解法提取牛皮中胶原蛋白各自的优缺点后,研究并最终确定了一种提取牛皮中胶原蛋白的新方法——酸酶复合法,该法工艺简单,在相对较短的制备时间内,胶原蛋白的提取率可以达到54.5%,同时又保证了胶原蛋白的天然三螺旋结构。同时以对胶原蛋白性质的研究结果为基础,建立一种快速且准确的胶原蛋白和明胶的鉴定及质量评价方法——快速鉴定评价法,包括聚丙烯酰胺凝胶电泳、丹宁酸沉淀实验、胰蛋白酶敏感性实验和体外成纤维能力实验。酸酶复合法和快速鉴定评价法对胶原蛋白提取工艺的大规模生产应用及对市场上各种产品的鉴定及品质评价具有实际意义。Generally,collagen is extracted mainly from animal skin under the aid of acids or enzymes.However,these methods usually have disadvantages of lower yield,time-consuming extraction,rigorous experimental requirements,and broken triplehelix structure.Based on the consideration of advantages and disadvantages of acid and enzymatic extraction methods,a novel approach,acid-enzyme combined method,for separating type I collagen from bovine skin was developed.This method provided a convenient process and a high yield of 54.5% within short time.In addition,products obtained could remain the natural triple helix structure.Meanwhile,a new method for identification and quality evaluation of collagen and gelatin designated as speedy identification and evaluation method was established based on qualitative and structural analysis of collagen.Therefore,the acidenzyme combined method and the speedy identification and evaluation method can offer promising practical applications in industrial production of collagen,and identification and evaluation of various commercial collagen products.

关 键 词：胶原蛋白 明胶 丹宁酸 三螺旋结构 鉴定 

分 类 号：TQ936.216[化学工程]