暴马丁香抑菌活性、抗氧化作用研究及微量元素的测定  

Ethanol Extract of Syringa reticulate:Evaluation of Antibacterial Activity and Antioxidant Activity and Analysis of Trace Elements

作　　者：张崇禧[1] 蔡恩博[2] 陈艳[2] 李金平[2] 黄坤[2] 关键[2] 薛长春[2] 郑友兰[2] 

机构地区：[1]山东大学威海分校海洋学院,山东威海264209 [2]吉林农业大学中药材学院,吉林长春130118

出　　处：《食品科学》2010年第7期131-134,共4页Food Science

摘　　要：目的:对暴马丁香的抑菌活性、抗氧化作用进行研究,并测定其微量元素的含量。方法:以暴马丁香茎皮80%乙醇提取物为材料,采用滤纸片法对5种常见细菌的生长抑制活性、最小抑菌浓度(MIC)进行测定,并讨论温度、pH值及紫外线对提取物抑菌效果的影响。采用DPPH法测定其抗氧化作用;采用原子吸收分光光度法测定其微量元素Fe、Mn、Zn、Cu的含量。结果:对金黄色葡萄球菌的MIC为1.563mg/mL,对沙门氏菌和巴氏菌的MIC均为6.25mg/mL。高温、酸性环境等对抑菌作用均无明显影响。其提取物50%抑制浓度(IC50)为0.042mg/mL。微量元素含量Fe为880.5μg/g、Mn为26.03μg/g、Zn为60.9μg/g、Cu为10.13μg/g。结论:暴马丁香茎皮有着良好的抑菌效果及较强的抗氧化作用,其微量元素含量较高,具有很好的开发价值。Objectives：To evaluate the antibacterial activities of 80% ethanol extract of Syringa reticulate stem bark against 5 common bacteria and its antioxidant activity and analyze trace elements.Methods：The minimal inhibitory concentrations（MICs） of Syringa reticulata extract against bacteria tested were determined by filter paper method.The effects of temperature,pressure,pH and ultraviolet irradiation on antibacterial activity of the extract were also investigated.DPPH free radical scavenging assay was used for antioxidant activity evaluation.The contents of trace elements such as iron,manganese,zinc and copper were determined using atomic absorption spectrophotometry.Results：The MICs of the extract was 1.563 mg/mL against Staphylococcus aureus,and 6.25 mg/mL against both Pasteurella and Salmonella enterica.The extract exhibited stable antibacterial activity when exposed to high temperature,ultraviolet light and acidic environment.The EC50（median effective concentration） of the extract for scavenging DPPH free radicals was 0.042 mg/mL.The contents of trace elements such as Fe,Mn,Zn and Cu were 880.5,26.03,60.9μg/g and 10.13 μg/g,respectively.Conclusion：Syringa reticulata stem bark has an excellent antibacterial activity and antioxidant activity and exhibits high levels of trace elements.This demonstrates its promising development potential.

关 键 词：暴马丁香 抑菌 抗氧化 微量元素 

分 类 号：S58[农业科学—作物学]