D-101大孔吸附树脂分离纯化橘皮中的黄酮类物质  

Purification of Crude Total Flavonoids Extracted from Citrus Peel Using D-101 Macroporous Adsorption Resin

作　　者：胡志军[1] 郝利君[1] 王南溪[1] 陈建秋[1] 

机构地区：[1]中国药科大学分析化学教研室,江苏南京210009

出　　处：《食品科学》2010年第8期65-69,共5页Food Science

基　　金：江苏省环境工程重点实验室开放基金项目(KF2007006)

摘　　要：目的:研究D-101大孔吸附树脂分离纯化橘皮黄酮类物质的工艺条件。方法:采用静态和动态吸附-解吸附两种方法,以黄酮类物质吸附率和解吸附率为评价指标,考察橘皮提取液pH值、吸附液料比、静置吸附时间、洗脱液种类和洗脱液料比等影响因素。结果:D-101大孔吸附树脂分离纯化橘皮黄酮类物质的最佳工艺条件为提取液pH4.43,吸附液料比15:1(黄酮类物质溶液:大孔吸附树脂,mL/g)、静置吸附时间90min、洗脱液为95%乙醇溶液、洗脱液料比25:1(95%乙醇溶液:大孔吸附树脂,mL/g)。结论:该方法简单、可行,能够用来分离纯化橘皮中黄酮类物质。To propose an optimal purification process of total flavonoids in 70% ethanol extract from citrus peel,the static and dynamic adsorption-desorption performance of D-101 macroporous adsorption resin towards total flavonoids from citrus peel was investigated.Major process parameters including pH of sample solution（ethanol extract of citrus peel）,sample solution volume/D-101 resin weight ratio（mL/g）,standing time and kind and amount（desorption solvent volume/D-101 resin weight ratio,mL/g） of desorption solvent affecting either adsorption ratio or desorption ratio of total flavonoids were evaluated and their optimal values were determined to be pH 4.43,15：1,90 min,95% ethanol and 25：1,respectively.The method proved to be simple and feasible for purification of crude total flavonoids extracted from citrus peel.

关 键 词：橘皮 黄酮类物质 D-101大孔吸附树脂 静态吸附 动态吸附 

分 类 号：O623.54[理学—有机化学]