蜂胶提取物处理对柑橘诱导抗病性的影响  

Effect of Propolis Extract Treatment on Induced Disease Resistance against Blue Mold in Citrus Fruits

作　　者：杨书珍[1] 彭丽桃[1] 潘思轶[1] 姚晓琳[1] 徐晓云[1] 

机构地区：[1]华中农业大学食品科学技术学院,湖北武汉430070

出　　处：《食品科学》2010年第8期275-279,共5页Food Science

摘　　要：以柑橘为材料,对蜂胶提取物诱导柑橘抗青霉病的表达时间以及蜂胶提取物处理对柑橘果实抗病性相关物质及酶的影响进行研究。结果显示,蜂胶提取物处理后48h人工接种意大利青霉,柑橘所表现出的抗病性最强。蜂胶提取物处理增加了柑橘果皮中酚类物质的含量,提高了柑橘果皮中苯丙氨酸解氨酶(PAL)、过氧化物酶(POD)、多酚氧化酶(PPO)、几丁质酶(CHT)与诱导抗病性密切相关的酶的活性。这表明蜂胶提取物可增强柑橘果实的防御系统,降低果实贮藏期间的发病率,对柑橘表现出良好的保鲜效果。Citrus fruits were treated for 10 min with propolis extract dissolved in a small amount of absolute ethanol and diluted with water to a concentration of 0.1%,inoculated with Penicillium italicum suspension（1×106 CFU/mL） at different post-treatment time points and stored at 26 ℃.After 72 h storage,the inhibitory effect of propolis extract treatment against lesion diameter of citrus fruits inoculated with Penicillium italicum was measured to determine the optimal expression time for resistance to blue mold disease.Besides,the changes in biochemical and physiological parameters of treated citrus fruits were examined.Results showed that treated citrus fruits exhibited the strongest resistance to blue mold disease at 48 h post-treatment.Propolis extract treatment increased the content of total phenolic compounds and enhanced the activities of enzymes related to induced disease resistance such as phenylalanine ammonia-lyase,polyphenol oxidase,peroxidase,and chitinase in citrus fruit peels.This demonstrates that propolis extract treatment has the ability to strengthen the defense system and reduce the blue mold disease incidence in citrus fruits,thereby providing an effective approach for the fresh-keeping of citrus fruits.

关 键 词：蜂胶提取物 柑橘 抗病性 青霉病 

分 类 号：S896.6[农业科学—特种经济动物饲养]