双底物诱导脂肪酶构象的固定及对底物的吸附特性  

IMMOBILIZATION OF LIPASE CONFORMATION INDUCED BY DOUBLE SUBSTRATES AND ADSORPTION CHARACTERISTICS OF LIGAND

作　　者：宋锡瑾[1] 厉瑾[1] 王杰[2] 徐佳音[1] 

机构地区：[1]浙江大学化学工程与生物工程系,杭州310027 [2]浙江大学化学系,杭州310027

出　　处：《离子交换与吸附》2010年第2期97-102,共6页Ion Exchange and Adsorption

基　　金：国家自然科学基金(No.20276067)

摘　　要：以聚乙二醇400二丙烯酸酯和三羟甲基丙烷三甲基丙烯酸酯为原料,加入底物月桂酸和正丁醇对脂肪酶进行活性构象诱导,再经紫外光引发聚合、抽提和干燥后,获得了双底物诱导脂肪酶构象的聚合物（DSLP）,对DSLP在30-60℃对配体的吸附能力进行研究。结果表明,DSLP对月桂酸和正丁醇的吸附能力大于空白聚合物（BP）和固定化酶聚合物（ILP）,显示底物诱导使DSLP对其配体具有较好的吸附能力。升温有利于增加DSLP对配体吸附量,当温度从30℃升高到40℃时,DSLP对月桂酸的平衡吸附量由25.83mg/g提高到36.08mg/g,DSLP对正丁醇的平衡吸附量由16.82mg/g提高到20.46mg/g。但随着温度的进一步上升,吸附量增加不明显。Lipase, lauric acid and n-butyl alcohol were added into a copolymer system, which contained PEG 400-dimethacrylate, trimethylolpropane trimethacrylate. The double substrates lipase polymers （DSLP） of locking lipase conformation were obtained by ultraviolet irradiation and lauric acid, n-butyl alcohol were extracted by petroleum ether. The results indicated that DSLP’s adsorption capacity was far stronger than black carrier’s and immobilized lipase polymers （ILP）’s. The adsorption of DSLP in the different temperature was studied. When the temperature is increased, the quantity of adsorption also rises. Equilibrium adsorption amount of lauric acid was 25.83mg/g at 30℃ , while that was 36.08mg/g at 40℃ . And equilibrium adsorption amount of n-butyl alcohol on the DSLP was 16.82mg/g at 30℃ , 20.46mg/g at 40℃ , respectively. On further increasing the temperature, the adsorption capacity of DSLP rises observably.

关 键 词：脂肪酶 月桂酸 正丁醇 构象 吸附 

分 类 号：O647[理学—物理化学] Q55[理学—化学]