利用糖蜜发酵生产丁醇菌株的分离筛选及鉴定  

Isolation, Screening & Identification of Butanol-producing Strains by Molasses Fermentation

作　　者：裴建新[1] 左文朴[1] 庞浩[1] 黄志明[1] 黎贞崇[1] 韦宇拓[2] 黄日波[1,2] 

机构地区：[1]广西科学院国家非粮生物质能源工程技术研究中心,广西南宁530007 [2]广西大学微生物及植物遗传工程教育部重点实验室,广西南宁530003

出　　处：《酿酒科技》2010年第5期32-35,共4页Liquor-Making Science & Technology

基　　金：桂科攻0992022-1(桂科转09321071);广西大学开放课题J0804(科学院基本业务费09YJ17SW05)

摘　　要：高效利用糖蜜产生丁醇的菌株是低成本生产丁醇的基础。本研究从自然环境(牛粪、沼气池、土壤)中筛选具有利用糖蜜产生丁醇能力强的菌株。采样通过玉米培养基富集初筛分离获得12株生产丁醇能力较强的菌株。用8度糖蜜发酵复筛,有2株菌株丁醇产量较高,分别为8.57 g/L和8.45 g/L,丁醇比高达75%。将菌株分别命名为gxzp-5和gxzp-9,经过16S rDNA基因序列同源性分析初步鉴定,2株菌株都为拜氏梭菌(Clostridium beijerinckii)。The strains capable of using molasses efficiently to produce butanol is the base for low-cost butanol production. In this study, such strains were isolated from natural environment （dairy manure, biogas, and soil）. 12 strains with strong butanol-producing capability were preliminarily isolated through corn accumulation culture. And two strains were subsequently obtained by 8 Bx molasses fermentation and their butanol-producing yield were 8,57 g/L and 8.45 g/L respectively. The two strains were named as gxzp-5 and gxzp-9 respectively and they were confirmed as clostridium beijerinckii through 16S rDNA gene sequence homology analysis.

关 键 词：微生物 糖蜜 生物丁醇 鉴定 拜氏梭菌 

分 类 号：TQ923[轻工技术与工程—发酵工程]