嗜热侧孢霉源纤维素酶酶学性质研究  

Study on the Enzymatic Properties of Thermophilic Sporotrichum-producing Cellulase

作　　者：张辉[1] 

机构地区：[1]聊城大学生命科学学院,山东聊城252059

出　　处：《酿酒科技》2010年第5期44-46,50,共4页Liquor-Making Science & Technology

摘　　要：对嗜热侧孢霉所产纤维素酶的酶学性质进行研究。结果表明,CMC酶、FPA酶和棉花酶的最适反应温度分别为60℃、60℃和55℃,在55～65℃范围内酶的热稳定性较好。CMC酶、FPA酶和棉花酶的最适反应pH分别为5.4、5.4和4.8,在pH4.8～6.0范围内,酶活力较稳定,Ca2+、Mg2+、Zn2+、K+和Ba2+对酶具有激活作用,Cu2+、Fe3+、Na+、Ag+和Co2+对酶具有抑制作用。The enzymatic properties of cellulase produced by thermophilic sporotrichum were studied. The results showed that the optimum reaction temperature ofCMCase, FPAase and cotton lyase were at 60 ℃, 60 ℃ and 55 ℃ respectively and cellulase had good thermal stability within 55-65 ℃. The optimum reaction pH values of CMCase, FPAase and cotton lyase were 5.4, 5.4 and 4.8 respectively and cellulase had stable activity as pH value was between 8-6.0. Ca^2＋,Mg^2＋, Zn^2＋, K^＋ and Ba^2＋ had activation effects on celluase. However, Cu^2＋, Fe^3＋, Na^＋, Ag^＋ and Co^2＋ had inhibition effects on cellulase.

关 键 词：纤维素酶 酶争挂质 嗜热侧孢霉 

分 类 号：TQ925[轻工技术与工程—发酵工程]