抗性淀粉对氧化偶氮甲烷诱导大鼠结(直)肠癌过程中肠道功能及变性隐窝病灶的影响  

Effects of resistant starch on intestinal function and aberrant crypt foci during the development of rat colorectal cancer induced by Azoxymethane

作　　者：孟妍[1] 陈立勇[2] 徐贵发[1] 

机构地区：[1]山东大学公共卫生学院营养与食品卫生研究所,济南250012 [2]山东大学附属省立医院营养科,济南250021

出　　处：《山东大学学报（医学版）》2010年第4期23-26,共4页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金资助项目(30872096)

摘　　要：目的研究抗性淀粉(RS)对结(直)肠癌大鼠的肠道功能及变性隐窝病灶(ACF)的影响。方法将50只雄性Wistar大鼠随机分为阴性、阳性对照组及RS低、中、高剂量组。阳性对照组大鼠从实验第2周开始腹腔注射氧化偶氮甲烷(AOM),每周1次,连续2周。阴性对照组注射生理盐水。RS各剂量组致癌剂处理同阳性对照组,并分别自由摄食含7.6%、15.2%及22.8%RS的饲料。阴性和阳性对照组大鼠摄食普通饲料。实验期间每周称重1次,于第5周末和第7周末分别进行粪便pH及排便实验。第16周断头处死所有大鼠,取出结肠组织进行指标ACF的测定。结果与阳性对照组相比,RS各剂量组ACF数目、肠道转运时间(GTT)均明显减少(P<0.01),粪便pH也有所下降(P<0.05),差异有统计学意义。结论RS有利于肠道内的环境健康,降低ACF的发生,可能是预防结(直)肠癌发生的机制之一。Objective To study the effect of resistant starch （RS） on intestinal function and aberrant crypt foci （ACF） for colorectal cancer in rats. Methods A total of 50 male Wistar rats were randomly divided into 5 groups： the negative control group,the positive control group and three different RS added groups （RS concentration was 7. 6% ,15. 2% and 22. 8% ）. Rats in the positive control group were subcutaneously injected with azoxymethane （AOM） once a week in the second and third weeks. Rats in the negative control group were injected with saline. Animals in different RS added groups were given the same treatment as the positive control group and received the corresponding doses of RS. Rats in the control groups received ordinary feed. Body weights were recorded weekly throughout the study. Fecal pH was measured at the 5th weekend and the gastrointestinal transit time （GTT） measurement was carried out at the 7th weekend. Rats were killed on the 16th week. Colons of rats were removed to determine the ACF index. Results Compared with the positive control group,the three RS added groups had an obviously reduced number of ACF and GTT（P〈0. 01） . The fecal pH was significantly decreased due to the addition of RS to the diet （P〈0. 05）. Conclusion RS is beneficial to maintain intestinal health and can reduce the formation of ACF,which might be one of the mechanisms of preventing colorectal cancer.

关 键 词：抗性淀粉 结直肠肿瘤 变性隐窝病灶 大鼠 Wistar 

分 类 号：R151.3[医药卫生—营养与食品卫生学]