粪鬼伞多糖脱蛋白方法的研究  

Study on deproteinization from Coprinus sterqulinus Fr. polysaccharide

作　　者：解耸林[1] 王志兵[1] 刘洪霞[1] 

机构地区：[1]长春工业大学化学与生命科学学院,吉林长春130012

出　　处：《食品与机械》2010年第2期5-7,12,共4页Food and Machinery

基　　金：吉林省教育厅科技发展计划项目(编号:吉教科合字[200660])

摘　　要：探讨粪鬼伞多糖提取过程中的脱蛋白方法与工艺。以蛋白质去除率和多糖损失率为考察指标,比较Sevag法、酶法、三氯乙酸法、酶-Sevag法脱蛋白效果。结果表明,酶-Sevag法脱蛋白效果较好且多糖得率较高,脱蛋白的最佳工艺条件为:酶用量2%,温度40℃,脱蛋白时间2h,pH值5.0;Sevag试剂浓度20%,脱蛋白次数3次,在此条件下,蛋白质去除率和多糖损失率分别为70.95%和6.58%。Investigated the method of deproteinization in Coprinus sterqulinus Fr. polysaccharide extraction. The effect of deproteinization with different methods（such as Sevag, enzyme, TCA and enzyme-Sevag method）were evaluated in terms of the ratio of protein removal and polysaccharide loss. The result showed that the enzyme-Sevag method could be the best, with less protein remain and polysaccharide loss. The optimum technological conditions of deproteinization were： enzyme usage 2%, temperature at 40 ℃, pH value 5.0, deproteinization time 2 h, Sevag concentration 20%, deproteinization times 3 times. The ratio of deproteinization and polysaccharide loss were 70.95% and 6.58%, respectively. Conclusion：Enzyme-Sevag method could be used for Coprinus sterqulinus Fr. polysaccharide deproteinization.

关 键 词：粪鬼伞 多糖 脱蛋白 酶-Sevag法 

分 类 号：R284[医药卫生—中药学]