NTNG1基因多态性与精神分裂症的关系  

Association between NTNG1 Gene Polymorphism and Schizophrenia

作　　者：朱宇章[1] 杨焕[1] 贺佳[1] 郑超 刘盈[1] 

机构地区：[1]中国医科大学附属第一医院精神医学科,沈阳110001 [2]本溪市心理卫生医院心理科,辽宁本溪117000

出　　处：《中国医科大学学报》2010年第4期286-289,共4页Journal of China Medical University

基　　金：辽宁省教育厅科学研究计划项目(2004D183)

摘　　要：目的探讨中国北方汉族人群中NTNG1基因rs4132604、rs2218404、rs1373336基因多态性与精神分裂症的关系。方法采用聚合酶链式反应-限制性内切酶片段长度多态性(PCR-RFLP)的方法,检测中国北方汉族316例精神分裂症患者及311例健康对照者的基因型,应用遗传统计学方法进行等位基因型及等位基因频率分析、单倍型分析。结果病例组和对照组NTNG1基因的rs4132604、rs2218404、rs1373336基因型频数分布均符合Hardy-Weinberg平衡定律(P>0.05);rs4132604等位基因频率(χ2=7.86,P=0.01)和基因型分布(χ2=7.67,P=0.02)在病例组和对照组间存在显著性差异,提示该基因在中国北方汉族人群中同精神分裂症的发病明显相关。病例组与对照组比较,rs2218404、rs1373336等位基因频率和基因型分布未见显著性差异。在单倍型分析中,rs4132604与rs2218404中的GG(χ2=6.11,P=0.01)和TG(χ2=5.28,P=0.02)、rs2218404与rs1373336中的GT(χ2=5.38,P=0.02)和GC(χ2=4.72,P=0.03)、三者中GGT(χ2=13.40,P=0.00)和TGT(χ2=6.56,P=0.01),两组间存在显著性差异。结论NTNG1基因的rs4132604多态位点同精神分裂症存在关联。NTNG1基因的rs4132604、rs2218404、rs1373336构成的单倍型上存在同精神分裂症发病的相关位点。Objective To investigate the genetic association between the polymorphism of rs4132604 site,rs2218404 site and rs1373336 site on NTNG1 gene and schizophrenia in Han population from the north of China.Methods PCR-RFLP analysis was used to detect the genotype of NTNG1 gene in 316 patients and 311 controls.Inheritance statistics methods were used to analysis the genotype,allele frequencies and haplotype.Results The genotypic frequency of the NTNG1 gene did not deviate from Hardy-Weinberg equilibrium in both patient group and control group（P 〉0.05）.The allele and genotype frequencies for rs2218404 and rs1373336 were not significantly different between the patients and the controls.However,SNP rs4132604 showed significant association with schizophrenia（G〉 T,χ^27.86,P =0.01,genotype χ^27.67,P=0.02）.Significant haplotype differences between case and control groups were observed.It include GG（χ^26.11,P=0.01）,TG（χ^25.28,P =0.02）between rs4132604 and rs2218404,GT（χ^25.38,P =0.02）,GC（χ^24.72,P =0.03）between rs1373336 and rs2218404,GGT（χ^213.40,P =0.00,TGT（χ^26.56,P =0.01）between three SNPs.Conclusion Rs4132604 variations in NTNG1 may play a role in schizophrenia in the Chinese Northern population.The frequencies of haplotypes between the three SNPs have differences between case and control groups.

关 键 词：NTNG1基因 精神分裂症 遗传学 基因多态性 限制性片段长度 

分 类 号：R749.3[医药卫生—神经病学与精神病学]