应用重组PCR构建猪源膜联蛋白A2和增强型绿色荧光蛋白融合基因  

Construction of Fusion Gene of Enhanced Green Fluorescent Protein with Annexin A2 from Pig by Recombinant PCR

作　　者：陈江涛[1] 吉艳红[1] 王光祥[1] 尚佑军[1] 刘艳红[1] 刘湘涛[1] 

机构地区：[1]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室,国家口蹄疫参考实验室农业部畜禽病毒学重点开放实验室,兰州730046

出　　处：《西北农业学报》2010年第1期38-43,共6页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：“十一五”国家科技支撑计划重大项目(2006BAD06A03)

摘　　要：膜联蛋白A2是膜联蛋白多基因家族成员之一,在Ca2+存在时能与带负电荷的磷脂结合。最近研究显示,膜联蛋白A2在病毒感染方面发挥了重要作用。本研究应用RT-PCR和PCR方法分别获得了猪源膜联蛋白A2和增强型绿色荧光蛋白基因(EGFP)。然后用重组PCR技术成功构建了膜联蛋白A2-EGFP融合基因,为研究膜联蛋白A2在病毒感染细胞中的定位和表达,以及病毒和宿主细胞相互作用奠定了基础。Annexin A2 is a member of the multigene family of annexin proteins,which are characterised by binding to negatively charged phospholipids in the presence of Ca2＋.Recent studies show that annexin A2 playes a critical role in virus infection.Annexin A2 from pig and enhanced green fluorescent protein were amplified and gained by RT-PCR and PCR respectively.Then the fusion gene of enhanced green fluorescent protein with annexin A2 from pig was constructed by recombinant PCR.This work was necessary for the study on the localization and expression of annexin A2 in infected cell by virus,and alse laid the groundwork for the investigation of interaction of virus with host cell.

关 键 词：重组PCR 膜联蛋白A2 增强型绿色荧光蛋白 融合基因 

分 类 号：Q78[生物学—分子生物学] S858.28[农业科学—临床兽医学]