大肠埃希氏菌耐药性水平传递研究  

Horizontal Transfer of Antibiotic Tolerance between Escherichia coli Isolates

作　　者：只帅[1] 席美丽[1] 申进玲[1] 杨保伟[1] 王新[1] 孟江洪[1] 

机构地区：[1]西北农林科技大学食品科学与工程学院,陕西杨凌712100

出　　处：《西北农业学报》2010年第4期47-50,共4页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：长江学者讲座教授基金项目;西北农林科技大学留学回国人员基金项目

摘　　要：以实验室分离大肠埃希氏菌菌株E11-83(耐链霉素)和E6-203(耐萘啶酮酸)为供体及受体菌,研究供体及受体菌的含量比、接合培养介质、氧气量、抗生素诱导、接合时间、温度等因素对接合率的影响。结果发现,供体与受体菌比例为1∶8时,接合率最高,为2.4×10-4;有氧条件下膜接合率最高,为2.5×10-4;添加低质量浓度阿莫西林对于大肠埃希氏菌的接合率影响较小;培养16 h接合率达到最高,为3.5×10-4;接合温度27℃时接合率最高,为3.1×10-4。Factors involved in horizontal transfer of antibiotic resistance between Escherichia coli isolates and conjugation frequency were studied in this experiment.E.coli strains E11-83（resistant to streptomycin） and E6-203（resistant to nalidixic） were used as donor and recipient strains respectively to study the influences of donor/receptor ratio,culture time,temperature,culture medium,and antibiotic factors on conjugation frequency.The results showed that the highest conjugation frequency of 2.4×10-4 was observed at donor/receptor ratio of 1∶8;Membrane culture method under aerobic condition was most effective,and had a conjugation frequency of 2.5×10-4;the conjugation frequencies of culture method added with amoxicillin or not were 2.9×10-4 and 2.4×10-4 respectively.The highest conjugation ratio 3.5×10-4 was observed after culture for 16 hours.At 27℃,conjugation ratio was 3.1×10-4.

关 键 词：大肠埃希氏菌 接合 影响因素 

分 类 号：R446.5[医药卫生—诊断学]