源于驴血清白蛋白的肽促进骨髓造血细胞增殖的试验研究  

Study on the Proliferation of Bone Marrow Hematopoietic Cells Promoted by Peptide from Donkey Serum Albumin

作　　者：李昊[1] 罗土炎[2] 严晟旻[1] 高小尧[1] 沈晓妙[1] 张志善[1] 饶平凡[1] 

机构地区：[1]福州大学生物工程研究所,福州350002 [2]福建省农科院中心实验室,福州350013

出　　处：《中国食品学报》2010年第2期12-19,共8页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金资助项目(No.30973974)

摘　　要：目的:探讨源于驴血清白蛋白的生物活性肽LH-6对骨髓造血细胞的体内外促增殖作用。方法:从正常人骨髓标本中纯化骨髓有核细胞,在DMEM培养基中加入不同浓度的生物活性肽LH-6培养;用透射电镜观察增殖后的骨髓细胞超微结构;将不同浓度的生物活性肽灌胃失血性贫血小鼠模型7 d,考察其体内作用效果。结果:肽LH-6可促进骨髓造血细胞增殖,有浓度依赖性,最佳作用浓度为1μmol/L;其促进骨髓有核细胞的增殖率达70%(p<0.05);用透射电镜观察增殖后的细胞超微结构,没有发生变化,体内效果显示肽LH-6对促进白细胞、红细胞和血小板增殖有一定的作用,其中促进白细胞增殖超过30%。结论:肽LH-6促进骨髓造血细胞的增殖,属于正常造血,为造血干细胞的体外扩增提供一条新途径。肽LH-6可作为潜在的补血目标新药。Objective：To investigate the promoting action of peptide LH-6 on the bone marrow hematopoietic cells proliferation in vitro and in vivo.Method：Purified bone marrow cells（BMCs） from human bone marrow samples were cultured in 96-well plates with DMEM mediums containing 10% FBS and the bioactive peptides were put at different concentration,counting the cells in each well.Ultrastructure of cells,which were treated by LH-6,was observed by transmission electron microscope.Mouse models of hemorrhagic anemia were established and mice were treated with different concentration grade peptide for 7 days by oral administration.Result：Peptide LH-6 promotes bone marrow hematopoietic cell populations proliferation and it was up to 70%（p0.05） of untreated control without cytomorphosis and it’s optimal concentration was 1 μmol/L.The populations of WBC,RBC and PLT was increased with treatment of peptide LH-6 in vivo.Especially,the proliferation of WBC was over 50% of untreated control after 7 days.Conclusion： peptide LH-6 promotes hematopoietic cells proliferation,which suggests us that this peptide is a new drug for salvage anemia.

关 键 词：生物活性肽 骨髓造血细胞 增殖 超微结构 

分 类 号：S879.9[农业科学—畜牧兽医]