基于质谱技术研究黑色素瘤血清特异性O-糖链  

Mass spectrometry analysis of melanoma related O-glycans in serum

作　　者：白晨[1,2] 吴刚[1] 赵亮[3,4] 

机构地区：[1]复旦大学生命科学学院,上海200433 [2]上海商学院食品学院,上海200235 [3]复旦大学化学系,上海200433 [4]复旦大学生物医学研究院,上海200433

出　　处：《中国科学：化学》2010年第5期517-522,共6页SCIENTIA SINICA Chimica

基　　金：上海市教育委员会科研创新项目(10YZ212);国家自然科学基金项目(30900254)资助

摘　　要：对接种和未接种B16黑色素瘤细胞的C57小鼠进行血清O-糖链比较糖组学研究,寻找黑色素瘤血清特异性O-糖链.小鼠血清10μL,β-消除反应释放O-糖链.反应混合物经石墨化炭黑固相萃取小柱(GCC SPE)分离纯化后,用于MALDI-Qit-TOF-MS分析.通过Launchpad软件采集并输出质谱数据,MATLAB进行数据解析,找到了10个稳定出现的差异糖链质谱峰.利用串联质谱分析了其中5个主要差异糖链的结构.Glycomics comparison was carried out for screening melanoma serum biomarkers between C57 mouse with and without B16 implanted.O-glycans were released from 10 μL serum by β-elimination,purified by GCC SPE and analyzed by MALDI-QIT-TOF-MS.MS raw data was acquired and exported by Launchpad software.MATLAB was then applied for further data analysis.10 glycans were considered to be firmly changed after B16 implantation and 5 of them were picked for MS/MS for structure analysis.

关 键 词：质谱 糖组学 黑色素瘤 分子标志物 

分 类 号：R739.5[医药卫生—肿瘤]