大孔树脂对三孢布拉霉菌产番茄红素的吸附性能研究  

Study on Adsorption Properties of Lycopene from the Broth of Blakeslea trispora by Macroporous Resin

作　　者：李干禄[1] 吴启赐[1] 武振军[1] 石楠[1] 万红贵[1] 

机构地区：[1]南京工业大学生物与制药工程学院,南京210009

出　　处：《中国生物工程杂志》2010年第4期71-76,共6页China Biotechnology

摘　　要：为利用大孔吸附树脂分离纯化三孢布拉霉菌发酵液番茄红素,考察了4种大孔树脂(HP20,HP2MG,SP825,SP207)对番茄红素的吸附-解吸特性,筛选出最佳树脂。结果表明,HP20大孔树脂对番茄红素具有较好的吸附-解吸性能,且吸附迅速,能在1h内达到吸附平衡。用含55%和60%异丙醇的丙酮不连续梯度洗脱,回收率可达89.55%,重结晶后可获得纯度95%以上的番茄红素,经红外、质谱、核磁等鉴定为天然型番茄红素。该方法简单,可操作性强,极具工业应用价值。The potential of macroporous resin for separation and purification of lycopene produced by B.trispora were investigated.The adsorption-desorption properties for lycopene of the resins such as HP20,HP2MG,SP825 and SP207 were studied.The optimal resin for lycopene was screened and selected.The results expressed that HP20 resin had a better adsorption-desorption property for lycopene.It could quickly reach an adsorption balance in one hour.Purification of lycopene using packed bed column by HP20 resin was carried out using step gradient of 55% isopropyl alcohol in acetone followed by 65% isopropyl alcohol in acetone.A 89.55% recovery of lycopene was detected by HPLC.After further recrystallisation the purity of lycopene was above 95% and the structure was confirmed by IR,MS and 1H-NMR.The whole process was simple,easy to operate,with a good industrial application value.

关 键 词：三孢布拉霉菌 番茄红素 大孔树脂 不连续梯度洗脱 

分 类 号：Q819[生物学—生物工程]