生物转化—从全细胞催化到代谢工程  

Bioconversion—from Whole Cell Biocatalysis to Metabolic Engineering

作　　者：郭明[1] 胡昌华[1] 

机构地区：[1]西南大学药学院现代生物医药研究所,重庆400716

出　　处：《中国生物工程杂志》2010年第4期110-115,共6页China Biotechnology

基　　金：重庆市科技攻关重点项目(CSTC2009AB1029)资助项目

摘　　要：与传统的化学合成方法相比,利用生物的手段转化生产活性化合物及其衍生物无疑具有更大的吸引力。随着用于生物转化微生物种类的增多,生物转化的应用领域不断得到扩大。生物转化的发展经历了野生型全细胞催化,基因工程微生物全细胞反应,以及利用系统分析和代谢工程进行全局性调控等几个阶段。以下对这一发展趋势及相关研究的最新进展作一简要综述。Biocatalysis was employed to do chemical transformations on non-natural man-made organic compounds.Biological methods offer a true practical advantage over chemical synthesis.With the development of microbial strain involved in bioconversion,enzymes and proteins are increasingly being used as biocatalysts in the generation of products that have until now been derived using traditional chemical processes.Such products range from pharmaceutical and agrochemical building blocks to fine and bulk chemicals and,more recently,components of biofuels.Recombinant microbial whole-cell biocatalysis is a valuable optimization and modification approach for producing enantiomerically pure interemediates.Metabolic engineering based on the systems-level analysis of cells and organisms is now offering a new powerful way of designing and developing strains to improve the performance in biocatalysis.Recent advances and development strategies of bioconversion were highlighted here.

关 键 词：生物转化 全细胞催化 基因工程 代谢工程 

分 类 号：Q819[生物学—生物工程]