超声波辅助白灵菇真菌多糖的提取及旋光检测  

Ultrasonic wave Extraction and Polarimetric Determination of Crude Epiphyte Polysaccharides from Pleurotus Ferulae

作　　者：黄桂玲[1] 张俊杰[1] 李红霞[1] 

机构地区：[1]河北理工大学化工与生物技术学院,河北唐山063009

出　　处：《河北理工大学学报（自然科学版）》2010年第2期79-83,共5页Journal of Hebei Polytechnic University:Social Science Edition

摘　　要：研究了白灵菇子实体多糖的超声波辅助提取工艺。通过试验,确定的最佳工艺条件为:料水比1:40(w:v),提取温度90℃,提取时间4 h,超声时间为10 min,提取2次,采用Sevage法去蛋白,乙醇沉淀浓度80%,并确定纯净水为白灵菇多糖的最佳提取溶剂。实验采用了旋光检测技术,并同比色法进行了对比。The extraction processing of crude epiphyte polysaccharides from Pleurotus ferulae with ultrasonic wave was summarized in the article,which the optimum conditions are：water as extractant,extraction temperature 90℃, w：v = 1：40,extraction time 4 hours,ultrasonic time 10 minutes,twice extraction operation.Sevage method is utilized to separate the protein and 80%alcohol solution was used to precipitate the crude epiphyte polysaccharides. Polarimetry is selected to detect the experimental results,which were compared with the UV method.

关 键 词：白灵菇 热水浸提法 超声波辅助 真菌多糖 旋光检测 

分 类 号：Q939.11[生物学—微生物学]