油桐油体蛋白基因的克隆及序列分析  

Cloning and sequence analysis of full-length cDNAs encoding oleosins from Vernicia fordii

作　　者：龙洪旭[1] 谭晓风[1] 陈洪[1] 张琳[1] 胡姣[1] 

机构地区：[1]中南林业科技大学经济林育种与栽培国家林业局重点实验室,湖南长沙410004

出　　处：《中南林业科技大学学报》2010年第4期31-38,共8页Journal of Central South University of Forestry & Technology

基　　金：国家林业公益性行业科研专项"油桐种质资源收集与定向培养技术研究"(2009-04-023)

摘　　要：油桐油体蛋白与油桐脂肪酸储藏密切相关。通过构建油桐cDNA文库和EST文库,用分子生物学手段分离克隆出两个油桐油体蛋白基因的全长cDNA序列。通过分析表明,两个油体蛋白基因的全长cDNA分别为738 bp、838 bp,各包含1个完整的CDS,分别编码137个、154个氨基酸。分别命名为VF_oleⅠ,VF_oleⅡ。通过与其它物种的19条油体蛋白氨基酸序列比对,油桐油体蛋白基因分别与麻疯树oleosin 3和麻疯树oleosin 2的相似性最高,分别为84%、82%。通过软件预测表明,油桐油体蛋白等电点分别为10.315、10.335,都存在跨膜结构域,且存在信号肽剪切位点,中部都存在一个较长的疏水区,C末端都是以α-螺旋为主。The oleosin of Vernicia fordii is closely related to the storage of fatty acid.Two full length cDNAs of oleosin gene were isolated and cloned from developing seeds of V.fordii by the cDNA library and EST library-based molecular techniques.The two full length cDNAs were 738 bp and 838 bp.They contained a complete CDS（coding sequence） of 738 bp and 838 bp encoding 137 amino acids and 154 amino acids,respectively.They were named VF_ole Ⅰand VF_ole Ⅱ.Amino acid sequence aligenment with 19 oleosins from other species showed that the two oleosins of V.fordii displayed the highest similarity（90.3%,88.3%） to R.communis oleosin and J.curcas oleosin 2.The predicted isoelectric points of the two V.fordii oleosins were 10.315 and 10.335.A membrane spanning domain was present in the two genes.There was a large possibility of the existence of cleavage site for signal peptide with a long hydrophobic region in the central sequence and α-helix in C-terminal.

关 键 词：油桐 油体蛋白基因 全长CDNA克隆 序列分析 结构预测 

分 类 号：S794.3[农业科学—林木遗传育种] TQ646.1[农业科学—林学]