帕金森病相关蛋白质相互作用网络的构建  

Construction of protein-protein interaction network of parkinson＇s disease associated proteins

作　　者：肖智权[1] 唐夏[2] 郭纪锋[1] 何丹[1] 沈璐[1] 严新翔[1] 唐北沙[1] 

机构地区：[1]中南大学湘雅医院神经内科,长沙410008 [2]中南大学软件学院,长沙410006

出　　处：《中华神经医学杂志》2010年第5期480-486,共7页Chinese Journal of Neuromedicine

基　　金：国家“973”重点基础研究发展计划基金（2006bc500700）;国家高技术研究发展计划（2006AA02A4080）;国家自然科学基金（30370515、30570638、30770735）

摘　　要：目的 构建帕金森病（PD）相关蛋白质相互作用网络. 方法 应用生物学信息方法 ,采用HGNC、OPHID和UniHI数据库收集所有PD相关蛋白及与PD相关蛋白相互作用的蛋白;利用ProteoLens软件绘制PD相关蛋白质相互作用网络;根据每个蛋白质对PD相关蛋白质相互作用网络的贡献给与评分,得到网络中每个蛋白质的关联系数. 结果 得到一个包含463个PD相关蛋白和767对PD相关蛋白质相互作用的网络,网络聚集度为90.5%（P=0.008）;其中SNCA、PARK2、DRD2、HTRA2、NDUFV2、DJl、DRDI、DRD3、TRAPl和ND3关联系数高.提示它们在PD发病机制中存在重要作用;APP、UBE21、CLIC6和UBB也获得了较高的关联系数,说明它们也参与了PD的发病机制;筛选到一些可能与PD发病机制相关的新义蛋白.如FLNA、FREQ、BIRC7、EPB41、EPB41L1、GIPCI、GNAZ、GRB2、KCNJ9、MAPK1、BAG5、CYCS. 结论 成功地构建了一个科学的、实用的PD相关蛋白质相互作用网络.进一步证实了一些蛋白在PD发病机制中的作用,并发现了一些可能与PD发病机制相关的新义蛋白.Objective To construct the protein-protein interaction network of Parkinson＇s disease （PD） associated proteins. Methods PD-related proteins and their interaction proteins were collected by bioinformatics method from HGNC, OPHID and UniHI database. A protein-protein interaction network of PD associated proteins was designed by ProteoLens software; each protein was evaluated by its contribution to the network and the relevance scores were calculated, thus the coefficient of association of each protein in the network was noted. Results A protein-protein interaction network containing 463 PD associated proteins and 767 their interaction proteins was obtained with its index aggregation reaching 90.5% （P=0.008）. The relevance scores of SNCA, PARK2, DRD2, HTRA2,NDUFV2, DJ1, DRD1, DRD3, TRAP1 and ND3 were much higher than that of the others, which indicated their important roles in the pathogenesis of PD. The relevance scores of APP, UBE2I, CLIC6 and UBB were a little higher among all the preteins, indicating that they also participated in the pathogenesis of PD. Some novel proteins, such as FLNA, FREQ, BIRC7, EPB41, EPB41L1, GIPC1,GNAZ, GRB2, KCNJ9, MAPK1, BAG5 and CYC, may also involved in the pathogenesis of PD.Conclusion A scientific and practical protein-protein interaction network of PD associated proteins is successfully constructed, which further confirms the role of some proteins in the pathogenesis of PD.Some novel proteins that might involve in PD are obtained too.

关 键 词：帕金森病 蛋白质相互作用网络 生物信息学 

分 类 号：R742.5[医药卫生—神经病学与精神病学]