聚乙烯醇降解酶产生菌发酵培养基响应面法优化研究  

Optimization of the fermentation medium for Pseudomonas strain producing a poly(vinyl alcohol)-degrading enzyme based on response surface methodology

作　　者：王能强[1] 赵维俊[1] 梁芳[1] 李会东[1] 王栋[1] 

机构地区：[1]湖南科技大学生命科学学院,湘潭411201

出　　处：《环境科学学报》2010年第6期1158-1163,共6页Acta Scientiae Circumstantiae

基　　金：湖南省教育厅资助科研项目(No.09C380)~~

摘　　要：为了提高假单胞菌Pseudomonas.sp XT11-Z90S产聚乙烯醇降解酶的能力,用Plackett-Buman实验和响应面分析实验对影响产聚乙烯醇降解酶的9个因素,即发酵培养基的9种组成成分进行了研究,并探讨了影响产酶的主要因素和培养基的最佳组成.结果表明,影响产聚乙烯醇降解酶的3个重要组成为葡萄糖、酵母膏和硫酸铵,其最适浓度分别为0.64、0.22和1.45g.L-1.在最佳浓度和组成条件下,聚乙烯醇降解酶最高酶活为13.5U.mL-1,与优化前的12.1U.mL-1相比,提高了11.6%,且其降解率6h时可达到35.5%.Two level factorial designs of the Plackett-Burman type were constructed to optimize the fermentation medium components of a poly（vinyl alcohol）-degrading enzyme produced by strain Pseudomonas sp.XT11-Z90S.The results showed that the concentrations of glucose,yeast extract and（NH4）2SO4 greatly influence the activity of poly（vinyl alcohol）-degrading enzyme.The optimum concentrations of glucose,yeast extract and（NH4）2SO4,determined by response surface methodology,were 0.64,0.22 and 1.45 g·L^-1,respectively.Under the optimal conditions mentioned above,the poly（vinyl alcohol）-degrading enzyme activity is 13.5U·mL^-1,which was increased by 11.6% compared with the value before optimization.

关 键 词：聚乙烯醇降解酶 PLACKETT-BURMAN DESIGN 响应面 优化 

分 类 号：X703.1[环境科学与工程—环境工程]