水菖蒲内生菌分离与抗菌活性菌株筛选  

Screening and Inhibitory Activity of Endophytic Fungi from Acorus calamus L.

作　　者：周晓坤[1] 陈钧[1] 韩邦兴[1,2] 何佳[1] 刘长风[1] 

机构地区：[1]江苏大学药学院,江苏镇江212013 [2]植物细胞工程安徽省工程技术研究中心,安徽六安237012

出　　处：《食品科学》2010年第9期211-215,共5页Food Science

摘　　要：对水菖蒲内生真菌进行分离,并进行抗菌活性的筛选。采用改良氯化三苯四氮唑显色(TTC)微量稀释法筛选抗菌活性菌株。以18种常见导致食品腐败的细菌、酵母菌和霉菌为指示菌研究活性菌株的抗菌能力。结果显示,水菖蒲内存在广泛的具有抗菌活性的内生菌,占内生菌总数的40.7%,高活性菌株占内生菌总数的12.39%。其中S9菌株发酵液具有抗细菌、酵母菌和丝状真菌的广谱抗菌能力,是潜在的食品防腐抗菌资源。In order to isolate and screen endophytic fungi with inhibitory activity from Acorus calamus L.,active strains were screened through modified TTC micro-dilution method.MIC（minimum inhibitory concentration） and MBC（minimum bactericidal concentration） were detected using 18 food spoilage strains.Results indicated that 40.7% endophytes exhibited antimicrobial activity and 12.39% endophytes exhibited higher antimicrobial activity.In addition,strain S9 exhibited the broadspectrum antimicrobial activity to bacteria,yeast and mycelia fungi.Active endophytic strains from A.calamus L.are potential resources for food preservation.

关 键 词：水菖蒲 内生菌 抗菌活性 

分 类 号：Q933[生物学—微生物学]