The synergistic anti-asthmatic effects of glycyrrhizin and salbutamol  

作　　者：Yang YANG Qian SHI Ze LIU Ruo-jie LI Peng-wei PAN Yuan-yuan HOU Wan-ge LU Gang BAI 

机构地区：[1]College of Life Sciences, Nankai University, Tianjin 300071, China [2]College of Pharmaceutical Sciences, Nankai University, Tianjin 300071, China [3]Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, Department of Biochemistry and Molecular Biology, Graduate Program in Genetics, Molecular and Cellular Biology, University of Southern California, Los Angeles, CA 90033, USA

出　　处：《Acta Pharmacologica Sinica》2010年第4期443-449,共7页中国药理学报（英文版）

基　　金：Acknowledgements The work was supported in part by the National High Technology Research and Development Program of China （863 Program, No 2006AA020502）, and Grant No 07JCZDJC05000 from the Natural Science Foundation of Tianjin, China.

摘　　要：Aim： To investigate the efficacy of glycyrrhizin （GL） combined with salbutamol （SA） as an anti-asthma therapy.Methods： Rat lung beta2-adrenergic receptor （β2-AR） mRNA level was measured by real-time RT PCR. Intracellular cAMP accumulation was evaluated with a reporter gene assay. An in vitro acetylcholine-induced guinea pig tracheal strip contraction model was used to test the relaxing effects of GL and SA. The anti-inflammatory effects of GL and SA were tested using tumor necrosis factor-α-induced NF-κB transcriptional activation reporter assay, I-κB Western blotting and interleukin-8 ELISA. An in vivo guinea pig asthma model was used to prove further the synergistic effect of GL and SA. Results： GL （0.3 μmol/L） increased mRNA levels of β2-AR in vivo and the accumulation of cAMP in vitro. The combination of GL and SA also resulted in significant complementary anti-inflammatory effects via inhibition of NF-κB activation, degradation of I-κB and production of interleukin-8. A significant synergistic effect of the combination was detected both in vitro and in vivo in a guinea pig mode.Conclusion： The results demonstrate that GL and SA have synergistic anti-asthmatic effects and offer the possibility of a therapeutic application of GL in combination with β2-AR agonists in the treatment of asthma.Aim： To investigate the efficacy of glycyrrhizin （GL） combined with salbutamol （SA） as an anti-asthma therapy.Methods： Rat lung beta2-adrenergic receptor （β2-AR） mRNA level was measured by real-time RT PCR. Intracellular cAMP accumulation was evaluated with a reporter gene assay. An in vitro acetylcholine-induced guinea pig tracheal strip contraction model was used to test the relaxing effects of GL and SA. The anti-inflammatory effects of GL and SA were tested using tumor necrosis factor-α-induced NF-κB transcriptional activation reporter assay, I-κB Western blotting and interleukin-8 ELISA. An in vivo guinea pig asthma model was used to prove further the synergistic effect of GL and SA. Results： GL （0.3 μmol/L） increased mRNA levels of β2-AR in vivo and the accumulation of cAMP in vitro. The combination of GL and SA also resulted in significant complementary anti-inflammatory effects via inhibition of NF-κB activation, degradation of I-κB and production of interleukin-8. A significant synergistic effect of the combination was detected both in vitro and in vivo in a guinea pig mode.Conclusion： The results demonstrate that GL and SA have synergistic anti-asthmatic effects and offer the possibility of a therapeutic application of GL in combination with β2-AR agonists in the treatment of asthma.

关 键 词：ASTHMA β2-adrenergic receptor agonists GLUCOCORTICOIDS GLYCYRRHIZIN SALBUTAMOL synergistic effects 

分 类 号：Q78[生物学—分子生物学] S859.84[农业科学—临床兽医学]