脂多糖对核转录因子kB在人牙龈成纤维细胞中的激活作用及意义  

Activating effects and significant of Lipopolysaccharide to the activity of nuclear transcription factor-kB in human gingival fibroblasts

作　　者：陈铁楼[1] 许兵[2] 苏丹[3] 周以均[4] 吴织芬[4] 

机构地区：[1]解放军第411医院口腔中心牙周病科,上海200081 [2]同济大学化学系,上海200092 [3]同济大学医学院,上海200092 [4]第四军医大学口腔医学院牙周科,陕西西安710032

出　　处：《临床口腔医学杂志》2010年第4期217-219,共3页Journal of Clinical Stomatology

基　　金：海军后勤部重点课题(2000-3327);南京军区"十一五"课题(06MA08);上海市课题(虹卫0603-15)

摘　　要：目的:探讨核转录因子kB(NF-kB)在牙龈成纤维细胞(GF)中活性的变化及其在牙周病发生中的意义,探讨NF-kB在牙周病调控中的作用及途径。方法:采用免疫细胞化学染色法,观察NF-kB在GF中活性的变化。结果:以10μg/mL的LPS作为有效刺激浓度刺激GF,6h后NF-kB核转位达峰值;以10～100μg/mL浓度的LPS作用GF时,NF-KB激活程度不同,表明10～100μg/mL浓度的LPS是NF-kB激活的有效浓度。结论:健康牙龈组织GF细胞中NF-KB处于非激活状态,LPS可诱导人GF细胞中NF-kB激活发生核转位,LPS通过调控NF-KB的激活调节炎症因子对牙周组织的破坏。Objective：To investigate the regulatory mechanism of nuclear transcription factor-kB（NF-kB） in periodontal disease by observing the lipopolysaccharide to NF-kB activity in human gingival fibrocytes（GF） and its significant to periodontal diseases.Method：The changes of NF-kB activity in human GF were observed with immunocytochemical technique.Result：After being stimulated by effective concentration of LPS with 10μg/ml,nucleus translocation of partial NFkB occurred at 3 h.The peak was at 6h.Concentration of 10 μg/mL～100 μg/mL was probably effective concentration of LPS to stimulate the cells and presented the different activity.Conclusion：Nucleus translocation of NF-kB could occur in cultured human GF induced by LPS,the NF-kB pathway could make it possible for LPS to induce GF to synthesize and secrete the pathogenesis factors.

关 键 词：核转录因子KB 脂多糖 免疫细胞化学 激活 分子调控 牙龈成纤维细胞 

分 类 号：R781.4[医药卫生—口腔医学] Q813.1[医药卫生—临床医学]