Altered Taurine Release in Hypothalamus of Aquaporin-4 Knockout Mice  

作　　者：ZHANG Hong-guo XIAO Ying-hong YANG Long-fei SU Wei-heng GUAN Xin-gang WANG Yu FENG Xue-chao MA Tong-hui 

机构地区：[1]Membrane Channel Research Laboratory, Northeast Normal University, Changchun 130024, P. R. China

出　　处：《Chemical Research in Chinese Universities》2010年第3期406-408,共3页高等学校化学研究（英文版）

基　　金：Supported by the National Natural Science Foundation of China(Nos.30670477 and 30770493)

摘　　要：Taurine is concentrated in glial cells in the hypothalamus and released in an osmo-dependent manner through volume-sensitive anion channels. Released tanrine acts on glycine receptors on vasopressin neurons to control vasopressin secretion. Water channel AQP4 is abundant in astrocytes in osmosensory areas such as the supraoptic nucleus of hypothalamus. An HPLC-based method was established to quantify taurine release from isolated hypothalamus tissues in wildtype and AQP4 knockout mice. Under the basal condition, there was no difference in taurine release from AQP4^＋/＋ and AQP4^-/- hypothalamuses. Taurine release from AQP4^-/- hypothalamus under hypoosmotic stimulation was significantly lower than that from AQP4^＋/＋ mice. AQP4 expression in the glial cells of the hypothalamus may play an important role in osmoregulation oftaurine release and subsequent vasopressin secretion.Taurine is concentrated in glial cells in the hypothalamus and released in an osmo-dependent manner through volume-sensitive anion channels. Released tanrine acts on glycine receptors on vasopressin neurons to control vasopressin secretion. Water channel AQP4 is abundant in astrocytes in osmosensory areas such as the supraoptic nucleus of hypothalamus. An HPLC-based method was established to quantify taurine release from isolated hypothalamus tissues in wildtype and AQP4 knockout mice. Under the basal condition, there was no difference in taurine release from AQP4^＋/＋ and AQP4^-/- hypothalamuses. Taurine release from AQP4^-/- hypothalamus under hypoosmotic stimulation was significantly lower than that from AQP4^＋/＋ mice. AQP4 expression in the glial cells of the hypothalamus may play an important role in osmoregulation oftaurine release and subsequent vasopressin secretion.

关 键 词：AQUAPORIN-4 TAURINE HYPOTHALAMUS HPLC Precolumn derivatization 

分 类 号：Q51[生物学—生物化学] Q78