中国部分水产品副溶血性弧菌毒力基因的分布特征  

Distribution of Virulence Genes in Vibrio parahaemolyticus Isolated from Seafood in China

作　　者：李薇薇[1] 王晓英[1] 郭云昌[1] 

机构地区：[1]中国疾病预防控制中心营养与食品安全所,北京100021

出　　处：《中国食品卫生杂志》2010年第3期239-243,共5页Chinese Journal of Food Hygiene

摘　　要：目的了解中国部分水产品中副溶血性弧菌毒力基因的分布情况。方法通过聚合酶链反应测定从中国部分水产品中分离的192株副溶血性弧菌是否携带毒力基因tdh、trh,及种特异性基因tlh、toxR。结果192株实验菌株tdh全阴性,4株trh阳性,毒力基因携带率2.08%;而tlh、toxR的携带率均为100%。结论中国副溶血性弧菌水产品分离株毒力基因携带率非常低。Objective To study the distribution characteristics of virulence genes in Vibrio parahaemolyticus isolated from seafood in China.Method Using polymerase chain reaction to detect the virulence genes tdh and trh,and to detect the species-specific genes tlh and toxR in 192 experiment strains.Results There was no strain carrying tdh gene,and 4 stains carrying trh gene,accounted for 2.08% of strains in 192 food isolates.The strains carrying tlh and toxR genes were 100%.Conclusion The rate of carrying virulence gene in Vibrio parahaemolyticus from seafood isolates was very low in China.

关 键 词：副溶血性弧菌 基因 聚合酶链反应 溶血素类 

分 类 号：R155.5[医药卫生—营养与食品卫生学]