急性压力超负荷心脏局部碱性成纤维细胞生长因子和肾素血管紧张素系统的变化及其意义  

Change of intracardiac bFGF and RAS and its significance in course of acute pressure overload

作　　者：何作云[1] 冯兵[1] 周小波[1] 覃军[1] 文芳[1] 

机构地区：[1]第三军医大学新桥医院心内科,重庆400037

出　　处：《中华心血管病杂志》1999年第1期64-67,共4页Chinese Journal of Cardiology

基　　金：国家自然科学基金!No:39600041

摘　　要：目的探讨急性压力超负荷时心脏局部碱性成纤维细胞生长因子（ｂＦＧＦ）和肾素血管紧张素系统（ＲＡＳ）在心肌肥厚发病中的作用。方法采用Ｗｉｓｔａｒ大鼠腹主动脉部分结扎组（ＯＧ），并与假手术组（ＣＧ）对比检测６个时相点（０．５小时、４小时、１天、５天、１０天和３０天）的左心室重量指数（ＬＶＷＩ）、心肌局部ｂＦＧＦ和ＲＡＳ。结果（１）ＯＧ中ＬＶＭＩ第５天增高（Ｐ＜０．０５），第１０天和第３０天更明显（Ｐ＜０．０１）。（２）ＯＧ中心肌ｂＦＧＦ免疫反应特异性染色强度增加，半定量显示５天升高（Ｐ＜０．０５），３０天基本恢复正常（Ｐ＞０．０５）；ｂＦＧＦ　ｍＲＮＡ表达１天升高（Ｐ＜０．０５），其后类似ｂＦＧＦ。（３）心肌血管紧张素转换酶（ＡＣＥ）活性和血管紧张素Ⅱ（ＡｎｇⅡ）水平在术后０．５小时即升高（Ｐ＜０．０５），４小时明显增高（Ｐ＜０．０１），峰值在３０天；心肌ＡＣＥ蛋白及ＡＣＥ　ｍＲＮＡ表达均在术后４小时明显增高（Ｐ＜０．０５或＜０．０１），其后逐渐升高，峰值在３０天；５天及其后，ＬＶＭＩ与ＡＣＥ蛋白及ＡＣＥ　ｍＲＮＡ表达数量呈正相关。结论本研究提示，心肌ｂＦＧＦ参与急性压力超负荷致心肌肥厚的早期阶段，而心肌局部ＲＡ?Objective To investigate the role of intracardiac renin angiotensin system (RAS) and bFGF inpathogenesis of myocardial hypertrophy. Methods Left ventricular mass index (LVMI), intracardiac RAS andbFGF were determined at 0.5 h, 4h, 1day (1d), 5 days (5d), 10 days (10d) and 30 days (30d) afterabdominal aorta constriction in rats (OG) and compared with false operation rats (CG). Results (1) LVMIgradually increased from 5d to 30d in OG (P<0.01); (2) Counts of intracardiac bFGF immune reaction andbFGF mRNA expression were increased at 5d, were essentially normalized at 30d; (3) Intracardial ACE activity,angiotensin Ⅱ (Aug Ⅱ) concentration, ACE protein and ACE mRNA expression were propessively risen from 0. 5h or 4h to 30 d; (4) LVMI was positively correlated with the level of ACE protein and ACE mRNA expressionfollowing 5d. Conclusion bFGF may only play an importannt role in early stage of cardiac hypertrophy inducedby pressure overload while intracardiac RAS may take part in various stages of the myocardial hypertrophy.

关 键 词：压力 负荷 心肌肥厚 原位杂交 BFGF RAS 

分 类 号：R542.202[医药卫生—心血管疾病]