肠型胃癌原发灶与淋巴结转移灶癌细胞染色体异常特征的比较  

Comparison of Chromosomal Alterations Between Primary Lesions and Metastatic Lymph Nodes of Intestinal-type Gastric Carcinoma

作　　者：刘东[1] 刘秀萍[2] 

机构地区：[1]辽宁省肿瘤医院病理科 [2]复旦大学上海医学院病理系

出　　处：《中外医疗》2010年第16期16-18,共3页China & Foreign Medical Treatment

摘　　要：目的探讨胃癌发生淋巴结转移的机制,寻找和定位与胃癌转移相关基因。方法本研究应用比较基因组杂交技术分析和比较了15例肠型胃癌患者原发灶和其对应的淋巴结转移灶癌细胞染色体基因组改变特征。结果较常见染色体DNA拷贝数扩增的部位是8q,13q,20和7;较常见染色体DNA拷贝数丢失的部位是1p,17p,19,21q和22q。其中,有意义的发现是20q12-13扩增,21qcen-21丢失,4q丢失,14q22-ter丢失。结论本研究结果表明胃癌原发灶和淋巴结转移灶癌细胞染色体基因改变最显著的部位是20q扩增,及21q,4q和14q的丢失;提示在这些部位可能存在与胃癌淋巴结转移相关的基因。Objective To investigate lymph-nodes metastases mechanism of gastric carcinoma.To find and locate the related genes of gastric carcinoma metastasis.Methods 15 cases of intestinal-type gastric carcinoma and the corresponding lymph-nodes metastases were studied by comparative genomic hybridization.Results The site of common chromosome DNA copy number gain is 8q,13q,20 and 7;the site of common chromosome DNA copy number loss is 1p,17p,19,21q and 22q.One important finding is gain of 20q12-13,loss of 21qcen-21,4q,14q22-ter.Conclusion The remarkable site of chromosomal genes alterations between primary lesions and metastatic lymph nodes of gastric carcinoma is gain of 20q and loss of 21q,4q,14q.These sites may exist the related genes of lymph-nodes metastases in gastric carcinoma.

关 键 词：比较基因组杂交技术 胃癌 染色体 DNA拷贝数 

分 类 号：R735.2[医药卫生—肿瘤]