银杏叶提取物对黄曲霉毒素B1诱发Wistar大鼠肝癌的影响  

EFFECT OF GINKGO BILOBA LEAF EXTRACT ON HEPATOCARCINOGENESIS INDUCED BY AFLATOXIN B1 IN WISTAR RATS

作　　者：蒿艳蓉[1,2] 杨芳[2] 曹骥[2] 欧超[2] 李媛[2] 杨春[2] 段小娴[2] 苏建家[2] 

机构地区：[1]广西壮族自治区人民医院临床肿瘤中心放疗科,南宁530021 [2]广西医科大学附属肿瘤医院肿瘤研究所

出　　处：《现代预防医学》2010年第11期2124-2126,2129,共4页Modern Preventive Medicine

基　　金：李嘉诚基金会资助项目(教育部教外港函[2001]33号);广西研究生教育创新计划资助项目(2007105981002D13)

摘　　要：[目的]通过建立黄曲霉毒素B1(Aflatoxin B1,AFB1)诱发Wistar大鼠肝细胞癌(HCC)模型,用银杏叶提取物(extract of Ginkgo Biloba Leaf,EGb761)进行干预,观察EGb761对AFB1致大鼠HCC作用的影响。[方法]实验大鼠按体重随机分成3组:A组(AFB1组)、B组(AFB1+EGb761组)和C组(空白对照组)。于实验第14、28、42及55周给予肝活检并于第64周全部处死大鼠,观察大鼠肝脏γ-GT灶及HCC发生情况。[结果]在出癌前期,第42及第55周时,A组每个γ-GT阳性灶的面积分别为(7.95±0.30)mm2及(17.87±0.71)mm2,单位面积(cm2)灶的个数分别为0.35±0.006及0.26±0.004,灶总面积分别为(2.54±0.05)mm2/cm2及(4.68±0.12)mm2/cm2;B组在相同时段的相应指标,分别为(4.65±0.16)mm2及(9.03±0.35)mm2,(0.21±0.006)及(0.20±0.005),(0.97±0.03)mm2/cm2及(1.62±0.06)mm2/cm2。除了55周A、B两组的单位面积γ-GT阳性灶的个数无统计学差异外,各时段B组各项指标都显著小于A组(P=0.000)。两个实验组共有28只大鼠发生恶性肿瘤,对照组无肿瘤发生。B组HCC诱发率(7/26,26.92%)明显低于A组(19/25,76%)(P=0.000)。另外,HCC及其他肿瘤的发生时间,B组明显推迟。[结论]EGb761能明显抑制AFB1诱发大鼠肝的癌前病变及延迟癌前病变向癌发展。[Objective] To study the effect of the extract of Ginkgo Biloba Leaf （EGb761） on aflatoxin B1 （AFB1） induced hepatocarcinogenesis in Wistar rats. [Methods] Wistar rats were randomly divided into three groups. The experimental animals were injected with AFB1 through abdomen. The rats in group B were given with AFB1 and EGb761. Group C was normal control. All rats were performed liver biopsy during hepatocarcinogenesis at 14thw, 28thw, 42thw and 55thw, and were executed at 64thw. Gamma-glutamyl transpeptidase-positive hyperplastic cell foci （γ-GT foci） and histopathology of the liver tissue were ob- served. [Results] At 42th w and 55th w, the γ-GT focus area （mm2/ focus）, number of foci （number/cm2） and general area of foci （mm2/cm2） were 7.95±0.30 mm2 and 17.87±0.71 mm2, 0.35±0.006 and 0.26±0.004, 2.54±0.05 mm2/cm2 and 4.68± 0.12 mm2/ cm2 respectively in group A; and were 4.65±0.16 mm2 and 9.03±0.35 mm2, 0.21±0.006 and 0.20±0.005, 0.97±0.03 mm2/ cm2 and 1.62±0.06 mm2/cm2 respectively in group B; The γ-GT focus area （mm2/focus）, general area of foci （mm2/cm2） and number of foci （numbers/cm2） in group B were significantly smaller than that in group A at differently period except numbers/cm2 at 55th w. At the end of experiment, 28 cases of malignant tumors were developed in group A and B. No tumor was found in group C. The incidence of hepatocellular carcinoma （HCC） in group A （19/25, 76%） was significantly higher than that in group B （7/26, 26.92%） and C （0%）（P = 0.000）. In addition, the time when hepatocarcinogenesis happened was remarkably delayed in group B. [Conclusion] EGb761 has an effective inhibitor effect to hepatocarcinogenesis induced by AFB1 in rats. Accordingly, prophylactic interventions with EGb761 may represent practical means to prevent the development of HCC.

关 键 词：银杏叶提取物 黄曲霉毒素B1 肝癌 γ-GT阳性灶 大鼠 

分 类 号：R735.7[医药卫生—肿瘤]