堆放垃圾渗滤液水溶性有机物的荧光特性  

Fluorescence characteristics of dissolved organic matter from dumped waste leachate

作　　者：何小松[1,2] 席北斗[2] 魏自民[1] 李鸣晓[2,3] 苏婧[2] 耿春茂[1,2] 王威[1] 胡春明[1,2] 

机构地区：[1]东北农业大学生命科学学院,黑龙江哈尔滨150030 [2]中国环境科学研究院水环境系统工程研究室,北京100012 [3]东北农业大学农学院,黑龙江哈尔滨150030

出　　处：《中国环境科学》2010年第6期752-757,共6页China Environmental Science

基　　金：国家"973"项目(2005CB724203);国家自然科学基金资助项目(50878201);黑龙江省自然科学基金资助项目(C200901)

摘　　要：为揭示堆放垃圾渗滤液物质组成特性及其变化规律,采用三维荧光光谱,对4个不同垃圾堆场渗滤液水溶性有机物(DOM)进行了研究.结果显示,堆放垃圾渗滤液样品S1中含有2类蛋白荧光峰:类酪氨酸荧光峰和类色氨酸荧光峰,其他3个样品(S2、S3及S4)只含有类色氨酸荧光峰,此外还出现了类腐殖质荧光峰,且不同样品中该峰的数目、类型及位置均存在差异,显示渗滤液样品S1只含有类蛋白类物质,而其他3个样品除此之外还含有类腐殖质物质,且腐殖化程度各异.类蛋白类物质-Hg(II)配位研究显示,与类色氨酸荧光峰相比,类酪氨酸荧光峰更易受介质微环境改变影响;室温培养模拟研究显示,与类腐殖质物质相比,类蛋白类物质更易发生降解.三维荧光光谱可以有效表征堆场渗滤液DOM物质组成及其变化规律.In order to reveal the characteristics of composition of leachates from dumped waste and their variations,three-dimensional excitation-emission matrix fluorescence spectroscopy（3DEEM） were applied for dissolved organic matter（DOM） of leachates extracted from four different dumped waste.Leachate sample S1 contained two different kinds of protein-like fluorescence peak,i.e.,tyrosine-like and tryptophan-like fluorescence peaks.The other three samples（S2,S3 and S4）,however,in addition to tryptophan-like fluorescence,contained humus-like fluorescence peaks as well,and the number,type and location of the peaks were different in according to samples.Leachate samples S1 contained only protein-like matter,while the other three samples contained both humus-like matter and protein-like matter,and the degree of humification of humus-like matter varied in different samples.The complexation between the protein-like matter in sample S1 and Hg（II） indicated that,compared with tryptophan-like fluorescence peak,tyrosine-like fluorescence peak could be more easily impacted by the micro-environmental change;the results of culture experiments at room temperature demonstrated that,protein-like substance could be easily broken,while humus-like substance shown strong resistance to microbe.3DEEM could be effectively characterized the composition of DOM of leachates from dumped waste and their variations.

关 键 词：堆放垃圾 渗滤液 水溶性有机物 三维荧光光谱 

分 类 号：X705[环境科学与工程—环境工程]