见血青总生物碱的抑菌活性和抗氧化性研究  

Antimicrobial and antioxidant activities of total alkaloids of Liparis nervosa(Thunb.)Lindl.

作　　者：董艳芳[1] 李伟阳[1] 叶睿超[1] 王丽[1] 

机构地区：[1]四川大学生命科学学院,成都610064

出　　处：《四川大学学报（自然科学版）》2010年第3期669-673,共5页Journal of Sichuan University(Natural Science Edition)

基　　金：四川省科技厅支撑项目(2009FZ0059)

摘　　要：采用滤纸片法,以12种细菌和4种真菌为试供菌种,对见血青总生物碱进行体外抑菌活性实验;并通过DPPH法测定见血青总生物碱清除自由基能力.抑菌实验结果表明:见血青总生物碱对16种微生物的生长均有很强的抑制性.抗氧化实验表明,见血青总生物碱在0.5～100μg/mL的实验浓度范围内具有很强的清除DPPH自由基的活性,清除率为5%～93.5%,其清除DPPH自由基的活性显著高于同等浓度的BHT.The screenings of antimicrobial and antioxidant activities of total alkaloids of Liparis nervosa （Thunb.） Lindl. were individually evaluated against 12 bacterial and 4 fungi, using a paper diffusion method and DPPH radical-scavenging method. All tested microorganisms were inhibited by the total alkaloids. The total alkaloids about 0. 5 to 100 μg/mL exhibited antioxidation from 5% to 93.5%, it showed stronger radical-scavenging activity about the concentrations tested than that of BHT, which was used as a positive probe.

关 键 词：见血青 总生物碱 抑菌性 抗氧化性 DPPH 

分 类 号：Q946.88[生物学—植物学]