多环芳烃降解菌X20的鉴定及降解特性  

Identification and degrading characteristics of PAHs-degrading bacterial strain X20

作　　者：杜彦玲[1,2] 台培东[1] 施秋峰 李培军[1] 

机构地区：[1]中国科学院沈阳应用生态研究所,沈阳110016 [2]中国科学院研究生院,北京100049 [3]有色金属华东地质勘查局,南京210007

出　　处：《生态学杂志》2010年第6期1208-1212,共5页Chinese Journal of Ecology

基　　金：国家高技术研究发展计划项目(2007AA061101和2008AA06Z331);中国科学院知识创新工程重要方向项目(KZCX2-YW-446);国家环保部"全国土壤现状调查及污染防治项目";国家环保公益性行业科研专项资助项目(200809047)

摘　　要：从多环芳烃降解高效的混合菌群中分离筛选到1株多环芳烃降解菌X20,经形态观察和16SrRNA序列分析,属于假单胞菌(Pseudomonas sp.)。采用室内摇瓶培养的方法,研究了该菌在不同环境条件下对菲和芘的降解。结果表明:弱碱环境有利于菌株X20对菲和芘的降解,最适pH为8.0;葡萄糖对菲芘降解率的影响呈抛物线变化,当葡萄糖浓度为0.2%时,X20对菲和芘的降解达到最高;X20对菲和芘的降解率随其初始浓度的上升而降低,菲和芘在初始浓度为10、20和40mg.L-1时的7d降解率分别为56.3%、39.25%、29.75%和41.8%、29.55%、23.50%,芘对X20降解的抑制强度高于菲。本研究结果将为构建高效的多环芳烃降解菌群,提高多环芳烃原位污染土壤的生物修复效果奠定基础。A bacterial strain X20 was isolated from a mixed microflora which can effectively degrade polyeyclic aromatic hydrocarbons （PAHs）. This strain was identified as Pseudomonas sp. , based on its morphological observation and 16S rRNA sequence analysis. The study on the degradation of phenanthrene and pyrene by X20, and the effects of medium pH, PAHs initial concentration, and glucose content on the degradation potential of X20 showed that weak alkaline condition favored the degradation of phenanthrene and pyrene by X20, and the optimal pH was 8.0. The effects of glucose concentrations on the degradation changed as parabolic, and 0. 2% was the optimal glucose concentration. When the PAHs initial concentrations were 10, 20, and 40 mg · L^-1 , the degradation rates of phenanthrene and pyrene were 56.3% , 39.25% and 29.75%, and 41.8%, 29.55% and 23.50%, respectively, i. e. , the inhibiting intensity of pyrene to X20 was higher than that of phenanthrene. This study provided a basis for constructing an efficient PAHs-degrading strain and improving the bioremediation effect of PAHs- in situ contaminated soil.

关 键 词：多环芳烃 微生物降解 16S RRNA 假单胞菌属 

分 类 号：X172[环境科学与工程—环境科学]