从桂黔交界地区中华硬蜱中分离到莱姆病螺旋体  

Borrelia burgdorferi isolated from Ixodes sinensis in the border areas between Guangxi and Guizhou in China

作　　者：李永学[1] 王昭孝[2] 曾霞[3] 王树声[4] 余春[2] 周敬祝[2] 

机构地区：[1]浙江省医学科学院病毒病研究所,杭州310013 [2]贵州省疾病预防控制中心 [3]广西医科大学 [4]广西壮族自治区疾病预防控制中心

出　　处：《中国媒介生物学及控制杂志》2010年第3期238-240,共3页Chinese Journal of Vector Biology and Control

摘　　要：目的从桂黔交界地区捕获的蜱中分离莱姆病螺旋体,以探查广西壮族自治区(广西区)和贵州省莱姆病的传播媒介。方法用BSK-Ⅱ培养基从蜱中分离莱姆病螺旋体。用PCR扩增分离菌株的5S～23SrRNA基因间隔区,将PCR扩增产物克隆后测序,通过将测序结果与基因库中莱姆病螺旋体菌株的5S～23SrRNA基因间隔区进行同源性比较和分析,从而鉴定分离株的基因型。结果从捕获的中华硬蜱中分离出1株莱姆病螺旋体(命名为QLT1)。分离株经鉴定为Borrelia valaisiana基因型莱姆病螺旋体。结论中华硬蜱很有可能是广西区和贵州省莱姆病的传播媒介。Objective Borrelia burgdorferi were isolated from captured ticks in the border areas between Guangxi and Guizhou,in order to identify the vector of Lyme disease in Guangxi Zhuang Autonomous Region and Guizhou province.Methods The BSK-Ⅱ culture media were used to isolate B.burgdorferi from ticks.The 5S-23S rRNA gene spacer region of the isolates was amplified by the PCR,and the product was cloned and sequenced.The sequencing results were compared with the 5S-23S rRNA gene spacer region of the B.burgdorferi strains from the gene bank and analyzed for the homology to identify their genotypes.Results One B.burgdorferi was isolated from the captured Ixodes sinensis（named QLT1）.The isolate was identified as the genotype B.valaisiana.Conclusion I.sinensis is the likely vector of Lyme disease in Guangxi Zhuang Autonomous Region and Guizhou province.

关 键 词：莱姆病 伯氏疏螺旋体 中华硬蜱 基因型 

分 类 号：R377[医药卫生—病原生物学]