S1P5对食管癌细胞Eca109黏附能力的影响  

Effects of S1P5 on adhesion of human esophageal cancer cell line Eca109

作　　者：胡为民[1,2] 李丽[2] 唐恩洁[1] 敬保迁[2] 冯莉[2] 王朝莉[2] 任碧轩[2] 

机构地区：[1]川北医学院微生物学与免疫学教研室,四川南充637007 [2]川北医学院免疫学与分子生物学研究所

出　　处：《西部医学》2010年第6期987-990,共4页Medical Journal of West China

基　　金：教育部科学技术研究重点项目(No.209105);川北医学院重点实验室开放基金项目(No.KFJJ(08)-03)

摘　　要：目的研究S1P5对食管鳞癌Eca109细胞黏附能力的影响。方法从人正常食管黏膜上皮克隆S1P5基因,构建表达载体S1P5-EGFP,转染Eca109细胞,经G418筛选,获得S1P5-EGFP/Eca109稳定细胞株。以对照载体Control-EGFP转染的Eca109细胞作为对照,荧光显微镜下观察细胞的形态,用黏附试验评价其黏附能力。结果成功构建S1P5-EGFP和Control-EGFP载体并转染Eca109细胞获得稳定细胞株。S1P5表达于细胞膜并使细胞略呈梭形改变。S1P5过表达致Eca109细胞黏附能力明显低于对照(P<0.05),并且不受其配体1-磷酸鞘氨醇(S1P)的影响。结论 S1P5组成性抑制Eca109细胞的黏附能力,食管癌细胞可能通过下调S1P5的表达增强其黏附。Objective To investigate the effect of S1P5 on adhesion of human esophageal squamous cell carcinoma（ESCC） cell line Eca109.Methods Human S1P5 gene was cloned from normal esophageal mucosal epithelium.S1P5-EGFP vector was constructed and transfected into Eca109 cells.The S1P5 expressing Eca109 cell was designated S1P5-EGFP/Eca109.With Control-EGFP vector transfecting Eca109 cell as control,cell morphology and adhesion were analyzed by fluorescence microscopy and cell adhesion assay,respectively.Results S1P5-EGFP and Control-EGFP vectors,and their corresponding stably-transfected Eca109 cells were successfully constructed.S1P5 overexpressing Eca109 cells displayed spindle cell morphology and S1P5 was expressed on the plasma membrane.The cell adhesion of S1P5-transfected Eca109 cells was lower than control vector-transfected cells with or without sphingosine 1-phosphate（P0.05）.Conclusion The data demonstrate that S1P5 constitutively inhibits Eca109 cell adhesion.Esophageal cancer cells may down-regulate the expression of S1P5 to promote adhesion.

关 键 词：S1P5 食管癌 ECA109细胞 

分 类 号：R735.1[医药卫生—肿瘤]