佛波酯对人卵巢癌细胞株SKOV3细胞体外生长的影响  

Effects of 12-tetradecanoyl-phorbol-13-acetate on the morphology and proliferation of human ovary cancer cell line SKOV3

作　　者：龚萍[1,2] 李红霞[1,2] 张素梅[1,2] 

机构地区：[1]北京大学第九临床医学院 [2]北京世纪坛医院妇产科,北京100038

出　　处：《武警医学院学报》2010年第7期519-522,F0003,共5页Acta Academiae Medicinae CPAPF

摘　　要：【目的】探讨佛波酯(TPA)对体外培养的人卵巢癌细胞株SKOV3细胞增殖、形态、细胞周期的影响。【方法】试验设试剂对照组、肿瘤细胞阴性对照组及5种不同浓度的促癌剂实验组。用四甲基偶氮唑蓝(MTT)快速比色法分析TPA不同浓度及不同时间对人卵巢癌细胞株SKOV3生长的作用;光镜下观测细胞形态学的改变;透射电镜观察细胞超微结构的变化;流式细胞仪检测细胞的凋亡及细胞周期的变化率。【结果】TPA具有抑制人卵巢癌细胞株SKOV3细胞增殖的作用,呈剂量和时间依赖性。细胞周期发生G1→S期阻滞。【结论】TPA能抑制SKOV3细胞增殖,显著地抑制卵巢癌细胞SKOV3的体外生长。[Objective] This study was designed to explore the effects of TPA on the proliferation, morphology, cell cycle distribution and ultrastructure of human ovary cell line SKOV3. [Methods] Grouth inhibition rates of SKOV3 cells were measured with MTT method. Cell cycle distribution and apoptosis was determined by Flow-cytometry. Morphological changes and ultramicrostructure of cells were observed with inverted phase contrast microscope and transmission electron microscope. [ Results] TPA could inhibited cell proliferation of human ovary cancer SKOV3 cells in a does-and time-dependent manner. [Conclusions] TPA can inhibite cell proliferation of human ovary cancer skov3 cells and significantly inhibit the growth of ovary cancer cells.

关 键 词：人卵巢癌细胞株SKOV3 TPA 细胞增殖 形态 细胞周期 超微结构 

分 类 号：R737.31[医药卫生—肿瘤]