在HBV和(或)AFB_1诱发树鼩肝癌过程中肝组织ras癌基因的表达  

The Expression of ras Gene in the Liver Tissues of the Tree Shrews Exposed to Human Hepatitis B Virus and/or Aflatoxin B 1

作　　者：班克臣[1,2] 苏建家[1,2] 杨春[1,2] 黄国华 覃柳亮[1,2] 李瑗 罗小玲[1,2] 段小娴 严瑞琪[1,2] 

机构地区：[1]广西肿瘤防治研究所 [2]中山医科大学肿瘤防治研究所

出　　处：《实验动物科学与管理》1999年第1期4-7,共4页Laboratory Animal Science & Administration

基　　金：国家自然科学基金

摘　　要：为了解在人乙型肝炎病毒（ＨＢＶ）和／或黄曲霉毒素Ｂ１（ＡＦＢ１）诱发树肝癌过程中Ｐ２１在肝组织的表达情况，用免疫组化方法观察人ＨＢＶ＋ＡＦＢ１双因素组（Ａ组）、单因素ＨＢＶ组（Ｂ组）、单因素ＡＦＢ１组（Ｃ组）、及对照组（Ｄ组）实验第４４周、１０５周、１１９周动物肝活检组织Ｐ２１蛋白的表达情况。结果，至１１９周，累计肝组织Ｐ２１阳性动物百分率分别为３５３、５３、１３３、０。表明人ＨＢＶ与ＡＦＢ１可协同促进树肝组织Ｐ２１的表达。To study P 21 over expression related to hepatocarcinogenesis in the tree shrews solely infected with hepatitis B virus(HBV)(group A), solely exposed to aflatoxin B 1(AFB 1)(group B), both infected with HBV and exposed to AFB 1(group C), control group(group D). Using immunohistochemical technique, the P 21 was investigated in tree shrew liver tissues by biopsies at 44th 104th 119th weeks. Untill 119th week, the accumulative total detecting rates of liver tissues in group A B C D were 35.3% 5.3% 13.3% 0%, respectively. The result suggested that exposure to HBV and AFB 1 may play a synergistic role in the expression of ras gene in the tree shrews.

关 键 词：树QU 肝癌 乙型肝炎病毒 黄曲霉毒素B1 

分 类 号：R735.7[医药卫生—肿瘤]