高效液相色谱法测定蔬菜中B族维生素  

Determination of vitamin b group in vegetables by high performance liquid chromatography

作　　者：谢玥[1] 贾丽[2] 焦玉海[1] 

机构地区：[1]北京石油化工学院,北京102617 [2]北京市理化分析测试中心,北京100089

出　　处：《中国食品添加剂》2010年第3期218-222,共5页China Food Additives

摘　　要：建立一种使用高效液相色谱与光电二极管阵列检测器（PDA）联用测定蔬菜中七种B族维生素的方法。色谱柱为ZORBAX SB-C18,以甲醇与缓冲溶液（庚烷磺酸钠、冰乙酸、三乙胺、EDTA、异丙醇）作为流动相进行梯度洗脱,设定267nm为检测波长。结果表明：VB1、VB2、VB6、VB12、叶酸、烟酸、烟酰胺的线性范围较宽,平均回收率为92.55%-116.71%,相对标准偏差在0.003%-0.013%,最低检出限能达到mg/kg级。本方法简单、实用,为测定蔬菜中水溶性B族维生素的含量提供了可靠依据。In this paper,seven vegetable＇s vitamin B was determined by high-performance liquid chromatography combined with a photodiode array detector（PDA）.The column was ZORBAX SB-C18,a methanol and buffer solution（sodium heptane,glacial acetic acid,triethylamine,EDTA,isopropyl alcohol）was the mobile phase in the gradient elution,and the detection wavelength was 267nm.The results showed that：VB1,VB2,VB6,VB12,folic acid,nicotinic acid,and nicotinamide had a wider linear range;the average recovery was 92.55%-116.71%,relative standard deviation was 0.003%-0.013%;the lowest detection thresholds was at the level of mg/kg.The method is simple and practical.It provided a reliable reference for the determination of water-soluble B vitamins in vegetables.

关 键 词：高效液相色谱 水溶性维生素 蔬菜 B族维生素 

分 类 号：TS202.3[轻工技术与工程—食品科学]