DJ-1蛋白在喉鳞状细胞癌中的表达及其与复发和转移的关系  

D J-1 expression in laryngeal squamous cell carcinoma and its relationship with tumor recurrence and metastasis

作　　者：祝小林[1] 文卫平[1] 雷文斌[1] 柴丽萍[1] 侯伟坚[1] 文译辉[1] 王仙仁[1] 

机构地区：[1]中山大学附属第一医院耳鼻咽喉科医院耳鼻咽喉科研究所,广州510080

出　　处：《中华耳鼻咽喉头颈外科杂志》2010年第6期497-501,共5页Chinese Journal of Otorhinolaryngology Head and Neck Surgery

基　　金：广东省自然科学基金（8151008901000153）

摘　　要：目的 探讨喉鳞状细胞癌（简称鳞癌）中DJ-1蛋白表达水平与喉鳞癌临床病理特征的关系.方法 将1995年1月至2006年1月收治的71例喉鳞癌患者术后肿瘤组织和9例非喉癌者喉黏膜组织应用免疫组织化学法检测,分析其DJ-1蛋白表达水平与临床病理特征的关系.结果 喉鳞癌组织中DJ-1蛋白阳性表达率为85.9%（61/71）,与非喉痛的喉黏膜组织55.5%（5/9）比较,差异有统计学意义（精确概率法P〈0.05）.分别就患者的性别、年龄、原发部位、T分期、临床分期、淋巴转移和肿瘤的病理学分级等情况进行分组比较,各组间喉鳞癌组织中DJ-1蛋白表达水平的差异均无统计学意义;DJ-1蛋白高表达患者其肿瘤复发率（53.3%）明显高于DJ-1蛋白低表达患者的复发率（26.8%;x2=5.164,P〈0.05）.Kaplan-Meier法和Cox回归分析发现喉鳞癌患者的肿瘤原发部位、性别、年龄、T分期、临床分期和肿瘤病理学分级对术后累积生存率影响均无统计学意义;DJ-1蛋白表达水平和淋巴转移对术后累积生存率影响有统计学意义（X2值分别为6.20和3.97,P值均〈0.05）.结论 DJ-1蛋白在喉鳞癌组织的表达水平高于喉黏膜组织.DJ-1蛋白表达水平较高的喉癌患者生存率可能低.Objective To detect the expression of DJ-1 in laryngeal squamous cell carcinoma （LSCC） and to study the relationship between DJ-1 expression and clinical indexes of LSCC. Methods The expressions of DJ-1 protein in 71 LSCC samples and 9 cases control samples from laryngeal mucosa tissues of non-LSCC patients were detected using streptavidin peroxidase immunohistochemistry stainin and the relationships between DJ-1 protein expression and clinicopathologic characteristics were analyzed. Results （1）The positive expression rate of DJ-1 protein in LSCC was 85.9% （61/71）, which was significantly higher than the rate （55. 5% ,5/9） in control laryngeal mucosa tissues （P 〈0. 05）. （2） DJ-1 expression was related to tumor recurrence （ P 〈 0. 05 ）, but not to sex, age, primary cancer position, T stage, clinical stage, lymph node metastasis and tumor differentiation. Tumor recurrence rate （53.3%）in the patients with higher expression of DJ-1 protein was higher than the rate （26. 8% ） in the patients with lower expression of DJ-1 protein （ X2 = 5. 164, P 〈 0. 05 ）. （ 3 ） With Kaplan-Meier curves and Cox regression analysis, the cumulative 5-year survival rates were correlated with D J-1 expression levels in laryngeal cancer tissues or cervical lymph node metastasis （ all P 〈 0. 05 ）, but not to sex, age, primary cancer position, T stage, clinical stage and tumor differentiation. Conclusions The expression of DJ-1 protein in LSCC is higher than that in control laryngeal mucous tissues. Overexpression of DJ-1 is associated with poor overall survival in LSCC patients.

关 键 词：喉肿瘤 癌 鳞状细胞 癌基因蛋白质类 细胞内信号肽和蛋白质类 免疫组织化学 

分 类 号：R739.65[医药卫生—肿瘤]